葡寡糖对LPS诱导小鼠氧化应激与炎症反应调节作用的研究  

Effects of Glucose Oligosaccharides on the Oxidative Stress and Inflammatory Response under LPS Induce

作　　者：刘云[1,2] 宋玉蓉[2] 乐国伟[1,2] 施用晖[1,2] 

机构地区：[1]食品科学与技术国家重点实验室江南大学 [2]江南大学食品学院食品营养与功能因子研究中心,无锡214122

出　　处：《天然产物研究与开发》2011年第2期240-245,共6页Natural Product Research and Development

基　　金：国家自然科学基金(30571347);科技部十一五支撑计划项目(2006BAD27B06)

摘　　要：本文研究了低、中、高三种不同聚合度葡寡糖(LGOS、MGOS、HGOS)对小鼠氧化应激与炎症反应的调节,并探讨其可能的作用机制。在采食正常日粮基础上,各组小鼠每日分别灌胃生理盐水、低聚果糖(FOS)、LGOS、MGOS、HGOS。21 d后,腹腔注射脂多糖(LPS)诱导小鼠建立氧化应激与炎症反应模型,分别测定LPS刺激后4 h和18 h时血清和肝脏中活性氧(reactive oxygen species,ROS)水平、总抗氧化能力(total antioxidant ca-pacity,T-AOC)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、丙二醛(Malondialdehyde,MDA)含量、肝脏中一氧化氮(NO)含量、一氧化氮合酶(nitric oxide synthase,NOS)活性、血清中白细胞介素1(Interleukin-1,IL-1)和肿瘤坏死因子(tumor necrosis factor,TNF-a)含量。结果表明:三种聚合度葡寡糖均能显著降低LPS刺激小鼠血清、肝脏中ROS水平(P<0.05),降低肝脏中NO含量、T-NOS、iNOS活性、血清中炎性细胞因子(IL-1、TNF-a)含量(P<0.05),显著提高总抗氧化能力(T-AOC)和抗氧化酶(CAT、GSH-Px)活性(P<0.05)。葡寡糖具有保护机体免受氧化损伤,减缓炎症反应的作用,并随着平均聚合度(degree ofpolymerization,DP)的增加,其调节功能逐渐增强。The effects of three different degrees of glucose oligosaccharides（LGOS,MGOS,HGOS） on the oxidative stress and inflammatory response were investigated and the mechanisms were also discussed.Mice of each group were treated with the normal saline,FOS,LGOS,MGOS and HGOS respectively,except feeding the ordinary diet every day.After 21 days,the model mice of the oxidative stress and inflammatory response under LPS induced was established.And the reactive oxygen species（ROS） level,total antioxidant capacity（T-AOC）,catalase（CAT）,glutathione peroxidase（GSH-Px）,malondialdehyde（MDA） in blood and liver,the content of nitric oxide（NO） and nitric oxide synthase（NOS） activity in liver,the content of Interleukin-1 （IL-1）,tumor necrosis factor（TNF-a） in blood plasma were detected after 4 hours and 18 hours.The results showed that LGOS,MGOS,HGOS could reduce the reactive oxygen species（ROS） levels in blood and liver,the content of NO,the activities of T-NOS and iNOS in liver and the contents of IL-1 and TNF-a in blood plasma（P0.05）,while increase the T-AOC and the activities of CAT and GSH-Px.Glucose oligosaccharides could protect the body from oxidative damage and slow down the inflammatory response and the regulatory function.In addition,the regulatory function was enhanced with the increase of average degree of polymerization.

关 键 词：葡寡糖 LPS 氧化应激 炎症 活性氧 抗氧化酶 

分 类 号：Q946.91[生物学—植物学]