大孔吸附树脂对苜蓿皂苷的吸附和解吸附作用(英文)  

Adsorption and Desorption of Alfalfa (Medicago sativa L.) Saponins by Macroporous Resin

作　　者：王蓟花[1] 周雨凡[2] 贺伟[2] 韩建国[3] 玉柱[3] 周立刚[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]北京林业大学林学院,北京100083 [3]中国农业大学动物科技学院,北京100193

出　　处：《天然产物研究与开发》2011年第2期275-278,共4页Natural Product Research and Development

基　　金：This work was co-financed by the grants from the Commonweal Fund of China Agriculture(nyhyzx07-022);the National Key Technology R&D Program of China(2006BAD16B08);the Introducing International Advanced Agricultural Project of the Ministry of Agriculture of China(2006-G38)

摘　　要：采用5种不同极性的树脂（AB-8、S-8、NKA-9、D-101和X-5）来评价对苜蓿皂苷的吸附和解吸附作用,其中中等极性的AB-8树脂对苜蓿皂苷具有最大的吸附量,用55%~65%的乙醇溶液能有效地将吸附的皂苷洗脱下来。当苜蓿粗提物量和AB-8树脂量为1∶1时,树脂的吸附量达到饱和。采用AB-8树脂,用90%乙醇洗脱,苜蓿提取物的最大解吸附量为108.4 mg/g干重树脂。通过大孔树脂吸附和解吸附,将90%乙醇洗脱液浓缩,皂苷含量（53%）是苜蓿粗提物含量（5.68%）的9倍。结果表明,AB-8大孔吸附树脂可用于苜蓿皂苷的大规模制备。Five types of macroporous adsorption resins namely AB-8,S-8,NKA-9,D-101 and X-5 with varying polarity were evaluated for the adsorption and desorption of alfalfa saponins.Of these,the moderately polar AB-8 resin showed the highest adsorption capacity and the absorbed saponins could be eluted effectively with 55-65% aqueous ethanol.The adsorption reached saturation at 1∶1 alfalfa extract to AB-8 resin mass ratio.The maximum desorption capacity of alfalfa extract was 108.4 mg/g dw AB-8 resin on a column eluted by 90% aqueous ethanol.The saponin content in the concentrated 90% aqueous ethanol effluent reached 53%,about 9 times of that（5.68%） in the crude alfalfa extract.The AB-8 macroporous resin has may be a favorable sorbent for large-scale saponin isolation from alfalfa.

关 键 词：紫花苜蓿 皂苷 大孔吸附树脂 吸附 解吸附 

分 类 号：Q946.8[生物学—植物学] S541[农业科学—作物学]