四种主要长春花生物碱超声波法提取工艺优化研究  

Optimizing the Extraction Process of Four Important Alkaloids from Catharanthus roseu by Ultrasonic Method

作　　者：张静[1] 隆小华[1] 刘玲[1] 刘兆普[1] 

机构地区：[1]南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室,南京210095

出　　处：《天然产物研究与开发》2011年第2期309-313,共5页Natural Product Research and Development

基　　金：国家海洋863资助项目(2007AA091702)

摘　　要：为探索高效、低污染的长春花中文多灵、长春质碱、长春新碱和长春碱的提取工艺,进行了超声波-甲醇法提取工艺条件的优化研究。结果表明:在单因素试验基础上,通过正交试验表明,超声波-甲醇法提取文多灵和长春质碱的最佳工艺条件为:提取溶剂:甲醇(pH4.0);料液比:1∶15;提取温度:45℃,提取时间:180 min,提取频率:59 KHz;文多灵和长春质碱平均得率为0.16%,0.08%。In order to explore the efficient and low contaminated extraction process of vindoline,catharanthine,vincristine and vinblastine from Catharanthus roseus,comprehensive optimization of condition in ultrasonic-methanol method were studied.On the basis of single factor experiments,orthogonal test was carried out to study.The optimum parameters for obtaining vindoline and catharanthine were determined as follows：methanol as solvent was used for 180 min,which pH value was 4.0.The ratio of raw material to solvent was 1∶15（w/v）,the extraction temperature was 45 ℃,and extraction frequency was 59 KHz.The yield of vindoline and catharanthine in the conditions was 0.164% and 0.081%,respectively.

关 键 词：超声波-甲醇法 正交实验 长春花 生物碱 提取工艺 优化 

分 类 号：Q946.88[生物学—植物学] TQ028.9[化学工程]