复合骨修复材料——聚氨基酸/硫酸钙的生物相容性研究  

Biocompatibility evaluation of a bone graft material-poly(amino acids)/calcium sulfate composite

作　　者：赵增辉[1] 蒋电明[1] 苏保[1] 代震宇[1] 权正学[1] 严永刚[2] 李鸿[2] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆400016 [2]四川大学物理科学与技术学院,四川成都610064

出　　处：《功能材料》2011年第5期807-811,共5页Journal of Functional Materials

基　　金：国家科技支撑计划资助项目(2007BAE13B00)

摘　　要：采用原位熔融缩聚法合成聚氨基酸/硫酸钙(poly(amino acids)/calcium sulfate,PAA/CS)复合材料。分别将PAA/CS圆片和其浸提液与L929细胞共培养后观察细胞形态变化,采用CCK-8试剂盒检测细胞增殖活性,流式细胞仪测定细胞周期。将致密圆柱状PAA/CS材料植入兔胫骨近端干骺端,进行大体和组织切片观察。结果表明材料对L929细胞生长活性无明显影响,细胞毒性0～1级,符合国家医用生物材料的细胞毒性要求。材料在动物体内不引起明显排异反应,并有新生骨长入材料内部,与宿主骨形成牢固的骨性愈合,具有良好的组织相容性和生物活性。PAA/CS composite was synthesized by melt polycondensation process.The PAA/CS plate and its extract were co-cultured with L929 cells for observation of the cells morphology.The proliferation activity of the L929 cells was quantified by a CCK-8 kit and the growth circle of the cells was determined on the flow cytometry.The compact PAA/CS cylinders were implanted into the proximal tibia in rabbits for macroscopical and histological observation.The results indicate that the PAA/CS composite and its extract has no influence on the L929 cell growth,and the cytotoxicity of PAA/CS composite is under level 1,which meets the national requirement for biomaterial.The PAA/CS composite is of excellent tissue compatibility and bioactivity that can guide new bone ingrowth and form bony bond with host bone,without any visible foreign body rejection.

关 键 词：骨修复 聚氨基酸/硫酸钙复合材料 细胞毒性 组织相容性 

分 类 号：R318.08[医药卫生—生物医学工程]