茉莉酸对PR-1、PR-2、PR-5和Ta-JA2基因表达以及小麦白粉病抗性的诱导  

Induction of PR-1,PR-2,PR-5,Ta-JA2 and wheat powdery mildew resistance in response to MeJA treatment

作　　者：牛吉山[1] 刘靖[2] 倪永静[1] 尹钧[1] 

机构地区：[1]河南农业大学国家小麦工程技术研究中心,郑州450002 [2]河南农业大学生命科学院,郑州450002

出　　处：《植物病理学报》2011年第3期270-277,共8页Acta Phytopathologica Sinica

基　　金：河南省杰出人才创新基金(0621001700);"十一五"国家科技支撑重大项目(2006BAD02A07)

摘　　要：为了探索小麦抗白粉病分子机理,明确茉莉酸对小麦白粉病抗性的诱导作用、对植物抗病性标志基因PR-1、PR-2、PR-5和本实验室克隆的1个新基因Ta-JA2的激活作用,以及抗病性变化与基因表达变化之间的相关性,本研究以感白粉病的小麦品种"中国春"、"濮麦9号"和"周麦18"为材料,用茉莉酸甲酯(methyl jasmonate,MeJA)喷洒小麦幼苗叶片进行诱导,通过离体叶段培养法接种白粉菌(Blumeria graminisf.sp.tritici,Bgt)进行抗性鉴定;用实时定量PCR技术检测小麦叶片中PR-1、PR-2、PR-5和Ta-JA2基因的表达变化。结果表明MeJA处理可以显著提高"中国春"、"濮麦9号"和"周麦18"对白粉菌的抗病水平。茉莉酸处理显著激活了PR-1、PR-2、PR-5和Ta-JA2的转录。茉莉酸诱导的抗病性提高与抗病标志基因PR-1、PR-2、PR-5及Ta-JA2的表达增强呈正相关。植物激素茉莉酸是小麦抗白粉病反应的信号分子。This study was carried out to determine the induction of jasmonic acid（JA） on powdery mildew resistance in wheat,the activation of the expression of plant disease resistant marker genes（PR-1,PR-2 and PR-5）as well as a new cloned gene Ta-JA2,and to investigate the relationship between the induced resistance and the gene expression patterns.Susceptible cultivars ＂Chinese Spring＂,＂Pumai 9＂ and ＂Zhoumai18＂ were used to study wheat resistance activated by methyl jasmonate（MeJA） and powdery mildew resistance was then assessed with detached leaf assay.Real time quantitative RT-PCR was used to determine the expression patterns of PR-1,PR-2,PR-5 and Ta-JA2.The results demonstrated that MeJA application enhanced the powdery mildew resistance of ＂Chinese Spring＂,＂Pumai 9＂ and ＂Zhoumai 18＂.The induced resistance could be detected from 12 h to 96 h after MeJA treatment,and the top value was at 24 h.MeJA significantly activated the expression of PR-1,PR-2,PR-5 and Ta-JA2.The induced powdery mildew resistance was positively associated with the induced expression of PR-1,PR-2,PR-5 and Ta-JA2.The phytohormone MeJA is a signal molecule in wheat powdery mildew resistance reaction.

关 键 词：小麦 白粉病 茉莉酸 抗性 基因 

分 类 号：Q945[生物学—植物学] S33[农业科学—作物遗传育种]