黄瓜枯萎病菌遗传多样性的AFLP分析  

AFLP analysis of genetic diversity of Fusarium oxysporum f.sp. cucumainum

作　　者：张艳菊[1] 陈霞[1] 刘东[1] 秦智伟[1] 周秀艳[1] 蒲子婧[1] 

机构地区：[1]东北农业大学,哈尔滨150030

出　　处：《植物病理学报》2011年第3期301-304,共4页Acta Phytopathologica Sinica

基　　金：国家科技部863项目(2006AA100108-4);"十一五"国家科技支撑计划项目(2006BA01D01A7);东北农业大学创新团队(CXT002-1-2);黑龙江省教育厅科学技术研究项目(11551058)

摘　　要：黄瓜枯萎病是由半知菌亚门尖孢镰刀菌黄瓜专化型（Fusarium oxysporumf.sp.cucumainumOwen）侵染引起的一种土传病害,是影响黄瓜生产的最主要病害之一。近年来随着分子生物学技术的迅速发展,国内外学者对于病原真菌的遗传多样性做了大量的研究,Wang等[2]对影响黄瓜枯萎病菌AFLP技术体系的多种因素作了探讨,得到了1种适合于黄瓜枯萎病菌AFLP分析的优化体系;Duan等[3]应用RAPD、The genetic diversity of Fusarium specises isolated from cucumber plants showing wilt disease from 8 cities was detected by amplified restriction fragment polymorphism（AFLP）.Sixteen combinations of primer pair were selected from 130 combinations and used to amplify the genomic DNA of 351 Fusarium oxysporum isolates.A total of 116 444 bands were obtained and 60 579 of them showed polymorphism,accounting for 52.0%.The genetic distances of 351 F.oxysporum isolates were ranged 0.56-1.00 based on AFLP markers,averaged at 0.78.The results showed that the genetic diversity among the F.oxysporum isolates was high.The 351 tested isolates could be classified into 5 AFLP groups,and most of the isolates from the same area were clustered in the same group by using the UPGMA method.

关 键 词：黄瓜枯萎病菌 AFLP分析 遗传多样性 AFLP技术体系 分子生物学技术 土传病害 尖孢镰刀菌 病原真菌 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]