结球甘蓝抗根肿病SSR分子连锁图谱的构建  

Construction of a molecular linkage map for clubroot resistance in cabbage using SSR markers(Brassica oleracea L.var.capitata)

作　　者：司军[1] 李成琼[1] 任雪松[1] 宋洪元[1] 宋明[1] 王小佳[1] 

机构地区：[1]西南大学园艺园林学院,重庆市蔬菜学重点实验室,南方山地园艺学教育部重点实验室,重庆400715

出　　处：《植物病理学报》2011年第3期305-309,共5页Acta Phytopathologica Sinica

基　　金：重庆市科技攻关项目(CSTC,2010AA1023);农业部公益性行业(农业)科研专项(nyhyzx07-007);西南大学博士基金项目(SWUB2008058);中央高校基本科研业务费专项资金项目(XDJK2010C068)

摘　　要：结球甘蓝（Brassica oleraceaL.var.capitata）简称甘蓝,是我国重要的蔬菜作物之一。甘蓝根肿病是由芸苔根肿菌（Plamodiophora brassicaeWoron.）侵染引起的一种世界性真菌病害。近年来,该病在我国的发病面积急剧增加,造成甘蓝产量和品质大幅度降低,选育抗病品种成为防治根肿病的重要途径之一。180 F2 individuals derived from the single cross between the resistant 15R and the susceptible 14S cabbage inbred lines were genotyped using simple sequence repeat（SSR）.Ninety-five primer pairs were used to detect the polymorphism between parents 15R and 14S,and 60（63.2%） of them showed polymorphism.The molecular linkage map was constructed with the 60 SSR primers.The results showed that 4 SSR markers were not linkaged;and 4 SSR markers had a segregation distortion;the other 52 SSR markers distributed among 9 linkage groups in cabbage spanning a 12.56 cM region of chromosome,and an average distance between markers was 11.9 cM.

关 键 词：结球甘蓝 分子连锁图谱 根肿病 SSR 蔬菜作物 真菌病害 抗病品种 根肿菌 

分 类 号：S635.1[农业科学—蔬菜学]