新疆西瓜花叶病毒2号遗传变异的PCR-SSCP分析  

Detection of genetic variations among Xinjiang isolates of WMV-2 by PCR-SSCP analysis

作　　者：黄素芳[1] 向本春[1] 李虎 刘升学[1] 安仙丽 

机构地区：[1]石河子大学农学院植物保护系,石河子832003 [2]自治区植物保护站,乌鲁木齐830006 [3]新疆油田公司白碱滩物业管理公司,克拉玛依834008

出　　处：《植物病理学报》2011年第3期310-313,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(30860168)

摘　　要：新疆地域广阔,各地气候条件差异大。西瓜、甜瓜在新疆种植面积较广,其病毒病的发生和危害较重。西瓜花叶病毒2号（Watermelon mosaic vi-rus2,WMV-2）是致病的主要毒源之一。前人对WMV-2的研究仅限于病原鉴定以及它对瓜类氨基酸的影响,而对它的遗传变异未做研究。为了深入研究新疆不同地域WMV-2致病性的差异,有必要弄清楚新疆的WMV-2有无遗传变异的发生。Twelve isolates of Watermelon mosaic virus 2（WMV-2） were collected from Cucurbitaceae plants in different areas of Xinjiang Uygur Autonomous Region in 2008,and their genetic variations were analyzed by RT-PCR-SSCP.A specific fragment of about 421 bp was amplified among all the isolates by RT-PCR.Using analysis of single-strand conformation polymorphism（SSCP）,12 isolates of WMV-2 were classified as five variant types： KS2（typeⅠ）,S7（type Ⅱ）,S4（type Ⅲ）,KS1 and S6（type Ⅳ）,M1,M2,K1,S3,S5,S1 and S2（type Ⅴ）.It was shown that there were genetic variations and differences in regional distribution among the isolates of Cucurbitaceae WMV-2 in Xinjiang,and there was obvious differentiation among the isolates of the different Cucurbitaceae plants in the same place.

关 键 词：西瓜花叶病毒2号 PCR-SSCP分析 遗传变异 新疆 WMV-2 MOSAIC 气候条件 种植面积 

分 类 号：S435.622.1[农业科学—农业昆虫与害虫防治]