RP-HPLC法同时测定人血清和脑脊液中苯巴比妥、苯妥英钠和卡马西平浓度  

Simultaneous Determination of Phenobarbital,Phenytoin and Carbamazepine in Human Serum and Cerebrospinal Fluid by RP-HPLC

作　　者：许贤瑞[1] 董通[1] 张庆[2] 杨小英[3] 党宏万[3] 

机构地区：[1]宁夏医科大学,银川75004 [2]宁夏医科大学附属医院神经内科,银川750004 [3]宁夏医科大学附属医院药剂科,银川750004

出　　处：《宁夏医科大学学报》2011年第4期360-363,共4页Journal of Ningxia Medical University

基　　金：宁夏自治区卫生厅重点科研项目(2009038);宁夏自然科学基金重点项目(NZ101120)

摘　　要：目的建立RP-HPLC法同时测定人血清和脑脊液中苯巴比妥(PB)、苯妥英钠(PHT)和卡马西平(CBZ)浓度的方法。方法色谱柱:Agilent ZORBAX SB-C18(250mm×4.6mm,5μm);流动相:甲醇-乙腈-水(27.5∶27.5∶45);流速:1.0mL.m in-1;检测波长:210nm;柱温:40℃;进样量:10μL。结果血清中PB、PHT、CBZ的线性范围依次为:2.64～52.75μg.mL-1、1.45～29.00μg.mL-1、1.04～20.80μg.mL-1;脑脊液中PB、PHT、CBZ的线性范围依次为:1.32～42.20μg.mL-1、0.73～23.20μg.mL-1、0.52～16.64μg.mL-1;血清中PB、PHT、CBZ的平均提取回收率分别为:93.17%、96.17%、94.67%,脑脊液中PB、PHT、CBZ提取回收率接近100%;日间、日内RSD均小于4%。结论本方法简单、快速、准确,适用于临床血及脑脊液浓度的检测。Objective To establish RP-HPLC method for simultaneous determination of phenobarbital（PB）,phenytoin（PHT） and carbamazepine（CBZ） in human serum and cerebrospinal fluid（CSF）.Methods Aglient ZORBAX SB-C18（250mm×4.6mm,5μm） was used.The mobile phase was water-methanol-acetonitrile（27.5∶27.5∶45）.The flow rate was 1.0 mL·min-1 and the detection wavelength was 210nm.The column temperature was 40℃ and sample size was 10μL.Results The linear relation of PB,PT and CBZ were 2.64～52.75μg·mL-1,1.45～29.00μg·mL-1,1.04～20.80μg·mL-1 respectively in human serum and 1.32～42.20μg·mL-1,0.73～23.20μg·mL-1,0.52～16.64μg·mL-1 in cerebrospinal fluid respectively.The average recovery rate of PB,PHT and CBZ were 93.17%、96.17%、94.67% respectively in human serum and nearly 100% in cerebrospinal fluid.The RSD for the inter-day and intra-day of three drugs were less than 4%.Conclusion The method is sample,fast,accurate and suitable for determination PB,PHT and CBZ in human serum and cerebrospinal fluid of drug concentration.

关 键 词：苯巴比妥 苯妥英 卡马西平 脑脊液 血清 高效液相色谱法 

分 类 号：R96[医药卫生—药理学]