绍兴鸭线粒体基因组全序列测定与分析  

Complete sequence determination and analysis of mitochondrial genome of Shaoxing duck

作　　者：涂剑锋[1] 司方方[1] 邢秀梅[1] 徐佳萍[1] 杨福合[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《浙江农业学报》2011年第2期268-272,共5页Acta Agriculturae Zhejiangensis

基　　金：国家科技支撑计划项目(2006BAD06B06)

摘　　要：参照近源物种线粒体基因组序列设计15对引物,通过PCR扩增、测序、拼接,获得绍兴鸭(Anas platy-rhychos)线粒体基因组全序列并进行序列分析。绍兴鸭线粒体基因组全长16 604 bp,碱基组成为29.20%A,22.19%T,15.78%G,32.83%C,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码控制区(D-loop),基因组成及排列顺序与其他鸟类相似,表明鸟类线粒体DNA进化上有较高的保守性。Using PCR amplification,sequencing and assembling,the complete mitochondrial genome of Shaoxing duck（Anas platyrhychos） was obtained.The full sequence was acquired from 15 primers designed according to close species＇ mitochondrial genome.The entire mitochondrial genome of Shaoxing duck was 16 604 bp in length,which contained 37 genes（13 protein coding genes,2 rRNA,22 tRNA） and a non-coding control region（D-loop）.The composition of the nucleotides A,T,G,C was 29.20%,22.19%,15.78%,32.83%,respectively.The gene composition and sequence arrangement was similar to other birds,indicating that mitochondrial DNA of bird was more conservative in evolution.

关 键 词：绍兴鸭 线粒体基因组 序列分析 

分 类 号：S834.89[农业科学—畜牧学]