极端环境分离的氧化微杆菌的嗜碱性及其基因组文库的构建  

Alkalophilicity and genomic library construction of Microbacterium oxydans isolated from an extreme environment

作　　者：吴小丹[1] 刘明[1] 王蔚[1] 万娟[1] 李爱英[1,2] 

机构地区：[1]华中师范大学生命科学学院,湖北武汉430079 [2]华中师范大学农药与化学生物学教育部重点实验室,湖北武汉430079

出　　处：《浙江农业学报》2011年第2期409-414,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(30770036);教育部高等学校博士学科点专项科研基金项目(20070511004)

摘　　要：从极端环境分离的微生物因适应特殊的生境而具有特殊的生理生化性质,已经成为重要的生物研究材料,而目前关于嗜碱氧化微杆菌的相关研究却非常少。为了解一株从新疆碱湖环境中分离的氧化微杆菌HSL10的生理特性以及进行其功能基因的克隆和分析,文章首先对菌株HSL10进行生长特性的研究,发现此菌株在培养基pH 5~12范围内生长良好,且生长较快;然后提取其总DNA建立基因组文库,重组率为100%。试验结果说明从碱性环境中分离的氧化微杆菌菌株HSL10具有嗜碱性,其基因组文库的构建可为相关功能基因的克隆和分析打下基础。Extremophiles feature in unusual physiological and biochemical characteristics due to adaptation to their extreme habitats detrimental to the majority of life on earth.Alkalophilic microbes gain greater attentions for scientific research and industrial applications,including the screening of alkalophilic enzymes,identification of novel drug candidates,clarification of the molecular mechanism of their alkalophilicity and characterization of bacterial diversity.Alkalophilic Microbacterium oxydans belonging to microbacteriaceae has been seldom studied.In the present study,A strain of HSL10 isolated from an alkaline lake in Xinjiang and identified previously as Microbacterium oxydans was observed to accumulate characteristic yellow-pigments,showed a rapid growth rate and grew well at a quite wide range of pH with optimal growth at pH 10,indicating that it is an alkalophilic microorganism.Additionally,a genomic library of HSL10 containing 1920 recombinants was constructed for further cloning and functional characterization of interesting genes located on the genome of HSL10.

关 键 词：极端环境微生物 氧化微杆菌 嗜碱性 基因组文库 

分 类 号：Q93[生物学—微生物学]