小鼠胸腺树突状细胞的分离和纯化方法的改进  

ISOLATION AND PURIFICATION OF MOUSE THYMIC DENDRITIC CELLS

作　　者：丁蓓蓓[1] 缪继武[1] 宋继志[2] 杨宁[1] 尹兰珍[2] 

机构地区：[1]南京铁道医学院组织学胚胎学教研室,南京210009 [2]南京铁道医学院,南京210009

出　　处：《解剖学报》1999年第4期381-383,共3页Acta Anatomica Sinica

摘　　要：目的　探讨一种简便易行的小鼠胸腺树突状细胞（ＴＤＣ）的分离纯化方法。　方法　密度梯度离心结合小鼠ＣＤ５ 单克隆抗体（ｍ Ａｂ）淘洗法（ｐａｎｎｉｎｇ）。　结果　细胞浓度在１．５×１０７ ／ｍ ｌ时进行Ｐｅｒｃｏｌｌ密度梯度离心可使分离纯度高、细胞丢失少；依次使用两种分离液分离的效果优于同一种分离液的反复使用；小鼠ＣＤ５单抗浓度为２５ｍ ｇ／Ｌ时，淘洗纯化效果较好，ＴＤＣ纯度达７５％ ～９０％ 。　结论　密度梯度离心与ＣＤ５单抗淘洗纯化的方法切实可行。Objective\ To explore a feasible method of isolating thymic dendritic cells (TDC). Methods\ Mouse thymic dendritic cells (TDC) were isolated from cell suspension by Ficoll Hypaque gradients and Percoll gradients of different densities and followed by panning procedure with various concentration of mouse monoclonal antibodies (mAb) to CD\-5. Results\ (1)In percoll gradients, cell concentration at 1 5×10 7/ml improvs TDC purity. (2)Two sorts of gradients used consequently may obtain higher purity than one sort of gradient used repeatedly. (3)Panning procedure with mouse mAb to CD5 concentration 25mg/L may obtain 75%\|90% purity of TDC. Conclusion\ Mouse TDC S were isolated and obtained by two sorts of gradients and panning procedure.\;

关 键 词：树突状细胞 胸腺 分离 纯化 小鼠 TDC 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]