金莲花种质遗传多样性的AFLP分析  

Study on Genetic Diversity of Trollius Chinensis Germplasms by AFLP

作　　者：李勇[1] 丁万隆 陈士林 朱殿龙 

机构地区：[1]中国医学科学院药 北京协和医学院用植物研究所,北京100193 [2]大连市药品检验所,大连116021

出　　处：《世界科学技术-中医药现代化》2011年第2期328-331,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家攻关项目(2004BA721A16):濒危药材金莲花及黄芩种质资源及其评价研究,负责人:丁万隆;科学技术部国家科技重大专项子课题(2009ZX09308-002):金莲花种子质量标准及金银花规范化种植绿色技术规范示范研究,负责人:李勇

摘　　要：金莲花是我国常用中药材品种,开展种质遗传多样性研究对新品种选育具有重要意义。本文利用AFLP标记技术对8份金莲花种质在基因组水平开展了遗传多样性分析,采用POP-GENE和NTSYS软件对统计结果进行遗传和聚类分析。从64对AFLP引物组合中筛选出的8对引物组合,能够区分全部供试金莲花种质;地理位置较近的金莲花种质,遗传相似系数较高;引种可造成金莲花种质的遗传结构发生变异。Trollius chinensis is one of the commonly used herbs in China. The study on genetic variation of T. chinensis has important significance for the breeding program. Genetic diversity of eight T. chinensis germplasms were analyzed by Amplified Fragment Length Polymorphisms （AFLP）. The genetic and phylogenetic statistic data were analyzed by the software of POPGENE and NTSYS. Finally, eight out of sixty-four AFLP primer combinations can be used to distinguish all T. chinensis germplasms tested in the study. The result indicated that T. chinensis in geographic regions at close range has higher genetic similarity. Therefore, the cultivation of T. chinensis in different areas may result in genetic variations.

关 键 词：AFLP 金莲花 遗传多样性 

分 类 号：R282.71[医药卫生—中药学]