补肾健脾中药复方对肾虚模型大鼠子宫内膜容受性的影响  

Influence of kidney-and-spleen-strengthening herbs on endometrial receptivity of kidney deficiency model rats

作　　者：曹蕾[1] 罗颂平[1] 欧汝强 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广东省计划生育研究所,广州510600

出　　处：《中华中医药杂志》2011年第5期1057-1061,共5页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:探讨补肾健脾中药复方对肾虚模型大鼠模型子宫内膜容受性的影响及其作用机制,并从"治未病"角度,研究补肾健脾中药复方之助孕3号方对不孕不育的作用及其机制。方法:以羟基脲建立肾虚模型大鼠,比较模型组、中药治疗组、空白组、中药治未病组大鼠动情期子宫内膜透射电镜下微细结构、内膜NK细胞亚群及LIF含量、血清性激素含量。结果:与模型组大鼠相比较,中药治疗组大鼠内膜腺上皮细胞表面微绒毛分布较密集,细胞内可见个别线粒体空泡化,内质网上可见较密集核糖体附着,细胞内可见分泌颗粒;动情期血清P含量明显提高(P<0.05),内膜LIF表达明显提高(P<0.05)。中药预防组大鼠内膜腺上皮表面微绒毛分布密集,细胞内见较多线粒体,内部结构清晰、正常,个别线粒体内可见空泡,粗面内质网分布密集,上见核糖体附着密集,细胞内可见分泌颗粒;中药预防组大鼠内膜各群NK细胞含量与正常大鼠无显著差异;中药预防组大鼠发情期血清E2、P与正常组无显著差异;发情期内膜LIF表达较模型组明显增多(P<0.05),与正常大鼠比较无显著差异。结论:补肾健脾中药复方能够有效改善肾虚模型大鼠子宫内膜腺上皮细胞功能,调节子宫内膜NK细胞亚群含量,提高内膜LIF表达,从而改善其子宫内膜容受性。Objective：To investigate the influence of kidney-and-spleen-strengthening herbs on endometrial receptivity of kidney deficiency model rats.Method：We used hydroxyurea to create kidney deficiency rat model.Compared the endometrial morphological and fine structure of endometrial,the percentage of different subsets of NK cells and the level of LIF in endometrial,of rats in estrus,among normal rats group,model without herbs group,model with herbal medicine group and normal rats with herbs group.Result：of the model with herbals medicine rat in estrus,the distribution of microvilli at the surface of endometrial epithelial was more dense.There were only a little vacuoles in mitochondria,there was more ribosomal densely attached to the surface of endoplasmic reticulum,and secretory granules could be saw in gland epithelial cells,compared with the model rat in estrus.The level of P in serum and LIF in endometrial of model with herbal medicine rats were both significantly higher than those of the model rats（P〈0.05）.And the content of LIF in endometrial of the normal rats with herbs was significantly more than that of the model group（P〈0.05）.Conclusion：Kidney-and-spleen-strengthening herbs could effectively improve the function of endometrial glandular epithelial cell of kidney deficiency model rats,adjust the function of NK cells,increase the content of LIF in endometrial,and then improve the endometrial receptivity.

关 键 词：补肾健脾 肾虚模型 子宫内膜容受性 

分 类 号：R285.5[医药卫生—中药学]