四神丸不同途径给药对溃疡性结肠炎大鼠结肠上皮细胞周期及脂质过氧化损伤的改善作用  

Therapeutic effect of Sishen Wan on lipid peroxidization injury in rats with experimental colitis treated by different ways of administration

作　　者：刘端勇[1,2] 管咏梅[3] 赵海梅[1] 程绍民[1] 万盼婷[1] 童文婷[1] 刘红宁[1] 

机构地区：[1]江西中医学院,南昌330025 [2]湖南中医药大学,长沙410208 [3]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中华中医药杂志》2011年第5期1168-1171,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中药新型给药系统技术平台十一五重大新药创制项目(No.2008ZX09310-005);江西省教育厅科学技术研究项目(No.GJJ09285);江西省卫生厅中医科研计划(No.2007A019;No.2009A005;No.2009A101);江西省卫生厅科技计划(No.20092050)~~

摘　　要：目的:观察四神丸不同途径给药抗脂质过氧化损伤治疗实验性结肠炎的疗效。方法:采用三硝基苯磺酸/乙醇复合法复制大鼠结肠炎模型,观察各大鼠体质量、结肠湿重、结肠重量指数、肉眼下结肠形态变化,光镜下病理变化及病理损伤评分,同时采用化学比色法检测髓过氧化物酶(MPO)活性,硫代巴比妥酸比色法(TBA),显色法检测丙二醛(MDA)活性,DTNB直接法检测谷胱甘肽过氧化物酶(GSH-Px)活性,嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性,以及流式细胞术检测结肠上皮细胞周期变化。结果:四神丸灌肠组可明显降低结肠炎大鼠结肠湿重、结肠重量指数、病理损伤评分,MPO活性、MDA含量及G0-G1期细胞数(P<0.05或P<0.01),而灌胃组则可降低结肠黏膜G0-G1期细胞数及MPO活性、MDA含量(P<0.05或P<0.01),其它则仅有下降趋势,但可显著性升高SOD水平(P<0.05)。灌胃与灌肠组间比较未见明显差异。各治疗组镜下均可见溃疡缩小、炎细胞浸润减少等修复表现。结论:四神丸可有效缓解实验性结肠炎结肠黏膜损伤,其可能的途径是抑制脂质氧化损伤,降低MPO活性和MDA含量,提高SOD水平,促进结肠上皮细胞修复,且灌肠给药略优于灌胃给药。Objective：To evaluate therapeutic effect of Sishen Wan on lipid peroxidization injury in rats with experimental colitis treated by different path administration.Methods：Therapeutic effect of Sishen Wan on UC rats were evaluated by colonic injury observed in macroscopic and microcosmic including morphologic change,pathological change,colonic injury score,and cell cycle of enterocyte analyzed by flow cytometry.Mechanism of Sishen Wan was explored in myeloperoxidase（MPO）,malondialdehyde（MDA）,glutathion peroxidase（GSH-Px） and superoxide dismutase（SOD）.Results：Compared with the model groups,many facets were remarkably decreased including colonic wet weight,colon weight index,colonic injury score,production of MDA,cellar number in G0-G1 in the Sishen Wan enema groups（P〈0.05 or P〈0.01）,and only the same statistical significance of MDA,MPO and cellar number in G0-G1 were found in oral Sishen Wan groups（P〈0.05 or P〈0.01）.And activity of SOD was markedly increased in in oral Sishen Wan groups（P〈0.05 or P〈0.01）.Meanwhile,the phenomenon of ulcer restored and the extent of inflammatory cell infiltration were observed in mucous membrane of colon from rats in Two Sishen Wan groups.Conclusion：Sishen Wan could effectively relieve colonic injury of rats with experimental colitis,which potential path was that Sishen Wan might inhibit lipid peroxidization injury,down-regulate contents of MDA and activity of MPO,up-regulate the level of SOD,improve colonic cytothesis to protect damaged colonic mucosa.And effect of Sishen Wan treated rats with experimental colitis by enema outweighed by intragastric administration.

关 键 词：四神丸 结肠炎 灌肠 抗氧化 疗效 

分 类 号：R285.5[医药卫生—中药学]