环介导等温扩增检测恶性疟原虫的应用研究  

Using loop-mediated isothermal amplification to detect Plasmodium falciparum infection in China

作　　者：陈勤[1] 张国庆[1] 郑彬[1] 沈玉娟[1] 田桢干[2] 曹建平[1] 徐馀信[1] 冯晓平[1] 何宇平[2] 汤林华[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025 [2]中华人民共和国上海出入境检验检疫局,上海200135

出　　处：《中国病原生物学杂志》2011年第4期269-272,共4页Journal of Pathogen Biology

基　　金：国家科技支撑计划项目(No.2009BAK43B31);国家科技重大专项(No.2008ZX10004-011)

摘　　要：目的探讨环介导等温扩增(loop-mediated isothermal amplification,LAMP)检测恶性疟患者血中疟原虫的敏感性和特异性。方法收集恶性疟患者和健康人血液样品,分别用LAMP、镜检法和巢式PCR检测恶性疟原虫,计算LAMP的敏感度、特异度、阳性预测值和阴性预测值,并与巢式PCR进行比较。结果共检测78份恶性疟患者和30份健康人血样,其中72份患者血样LAMP检测为阳性,以镜检法为金标准,LAMP筛检的灵敏度为92.3%,特异度为100%,阳性预测值和阴性预测值分别为100%和83.3%;以巢式PCR为参考,LAMP筛检的灵敏度为97.2%,特异度为94.4%。结论 LAMP方法检测恶性疟原虫的敏感度和特异度与巢式PCR方法相近,可应用于现场恶性疟检测。Objective To investigate the sensitivity and specificity of loop mediated isothermal amplification （LAMP） at detecting Plasmodium falciparum infection. Methods Blood samples of patients with malaria and health people were collected. Microscopic examination, nested PCR, and LAMP were used to detect P. falciparum. The sensitivity, specificity, 95% CI, positive predictive value （PPV）, and negative predictive value （NPV） of LAMP were calculated and compared to those of nested PCR. Results In total, samples were collected from 78 patients and 30 healthy individuals for microscopic examination. Of the 78 patients, 72 were positive according to LAMP. LAMP had a sensitivity of 92.3%and a specificity of 100% with microscopic examination as the golden standard. PPV and NPV were 100% and 83. 3%. LAMP had a sensitivity of 97.2% and specificity of 94.4% with nested PCR as a reference. Conclusion The sensitivity and specificity of LAMP were equivalent to those of nested PCR, so LAMP can he used in the field.

关 键 词：环介导等温扩增反应 疟原虫 恶性 18S RRNA基因 

分 类 号：R382.31[医药卫生—医学寄生虫学]