搜风祛痰法对血管紧张素Ⅱ诱导血管内皮细胞LOX-1 mRNA表达的影响  

Search Wind Removing Phlegm to Angiotensin Ⅱ Induced Vascular Endothelial Cell LOX-1 mRNA Expression

作　　者：周景慧[1] 吴敏[1] 宫丽鸿[1] 高峰[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2011年第5期1125-1127,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：沈阳市科技基金资助项目(1071215)

摘　　要：目的:观察搜风祛痰中药对血管紧张素II致体外培养人脐静脉内皮细胞LOX-1 mRNA表达的影响。方法:制备兔活心汤含药血清。采用酶消化法培养HUVECs 2-3代用于实验,采用RT-PCR法测定内皮细胞LOX-1 mRNA,随机分成5组:正常对照组,AngⅡ组,低浓度药物血清+AngⅡ组中浓度药物血清+AngⅡ组高浓度药物血清+AngⅡ组。结果:活心汤可抑制AngⅡ所致的内皮细胞损伤,减少LOX-1 mRNA的表达。结论:活心汤可抑制AngⅡ所致的HUVECs损伤,可以保护血管内皮功能。Objective ：To observe the search expectorant herbs on the wind induced by angiotensin II in cultured human umbilical vein endothelial ceils LOX - 1 mRNA expression. Methods ： Rabbit hearts decoction live. With enzyme digestion training HUVECs2 - 3 Generation for experiments using RT - PCR method for the determination endothelial LOX - 1 mRNA, were randomly divided into 5 groups： normal control group, Ang Ⅱ group, low concentration of serum AngⅡ group, the concentration of serum Ang Ⅱ group at Serum concentrations of Ang Ⅱ group. Results ： Live ）（in Tang inhibi- ted Ang Ⅱ - induced endothelial cell injury, reduced LOX - I mRNA expression. Conclusion ： Live Xin Tang HUVECs in- hibited Ang Ⅱ -induced damage, can protect endothelial function.

关 键 词：血管紧张素Ⅱ 内皮细胞 LOX-1 搜风祛痰 

分 类 号：R285.5[医药卫生—中药学]