嗜盐古菌Haloferax sp. H4蛋白质表达差异研究  

Study on the Protein Expression Difference of Halophilic Archaea Haloferax sp. H4

作　　者：刘杰[1] 高凯[1] 陈晨[1] 陈绍兴[1] 

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661100

出　　处：《安徽农业科学》2011年第13期7599-7600,共2页Journal of Anhui Agricultural Sciences

基　　金：红河学院生物化学与分子生物学重点学科建设项目(071010);红河学院校级课题项目(XJ1Y0802);云南省教育厅科学研究基金(09Y0413)

摘　　要：[目的]研究嗜盐古菌Haloferax sp.H4在不同环境中的蛋白表达差异。[方法]采用变性聚丙烯酰胺凝胶电泳(SDS-PAGE)检测不同NaCl浓度、pH、温度、有氧和无氧条件下的H4菌株蛋白表达差异。[结果]H4菌株在不同NaCl浓度、有氧和无氧条件下的蛋白表达存在明显特异性;而在不同pH和温度条件下,其蛋白表达差异性不明显。[结论]嗜盐古菌对不同环境因子的调控方式可能存在差异。[Objective] The research aimed to study the protein expression difference of halophilic archaea Haloferax sp.H4 under the different environments.[Method] SDS-PAGE was used to detect the protein expression difference of H4 strain under the different NaCl concentration,pH value,temperature,aerobic and anaerobic conditions.[Result] The protein expression of H4 strain had the obvious specificity under the different NaCl concentration,aerobic and anaerobic conditions.But under the different pH value and temperature conditions,the protein expression difference wasn＇t obvious.[Conclusion] Maybe the regulation manner of halophilic archaea on the different environmental factors had the difference.

关 键 词：嗜盐古菌 HALOFERAX sp.H4 蛋白表达差异 

分 类 号：S188[农业科学—农业基础科学]