圆鼻巨蜥遗传多样性的RAPD分析  

RAPD Analysis for Genetic Diversity of Varanus salvator

作　　者：傅美兰[1,2] 于冬梅[2] 王莹[1] 彭建军[2] 

机构地区：[1]暨南大学生命科学技术学院,广东广州510632 [2]华南濒危动物研究所物种鉴定中心,广东广州510260

出　　处：《安徽农业科学》2011年第13期7842-7845,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金项目(9151026001000003);广东省科学院优秀青年科技人才基金项目(200704);广东省野生动物保护和利用公共实验室基金项目(200901)

摘　　要：[目的]采用RAPD技术对圆鼻巨蜥（Varanus salvator）进行遗传多样性分析。[方法]用20个随机引物对圆鼻巨蜥36个个体的基因组DNA进行PCR扩增。[结果]有10对引物能扩出清晰稳定的条带,共扩增出2952条DNA片段,平均每个个体扩增出82个条带,其中47条具多态性,多态性位点比为57.32%,36个个体间遗传距离为0.035 9~0.335 9,平均遗传距离为0.135 92。Nei s基因多样性指数H=0.181 9,Shannon s多样性指数I=0.263 0,表明圆鼻巨蜥具有较高的遗传多样性。[结论]采用UPGMA法构建了36个个体相互关系的分子聚类图,发现没有形成明显的种群分化。[Objective] The aim was to carry out RAPD analysis on genetic diversity of Varanus salvator.[Method] 20 random primers were used for PCR amplification of genomic DNA of 36 individuals of V.salvator.[Result] 10 primers could produce highly reproducible RAPD bands.A total of 2 952 DNA fragments were successfully amplified.Each individual got 82 amplified bands on average,47 of which showed polymorphism.The polymorphic locus percent was 57.32%.The genetic distance among 36 individuals ranged from 0.035 9 to 0.335 9 with an average of 0.135 9.The Nei＇s gene diversity index（H） and Shannon＇s information index（I） were 0.1819 and 0.263 0,respectively,indicating that V.salvator had greater genetic diversity.[Conclusion] The phylogenetic tree was inferred by using UPGMA analysis,it was found that the 36 individuals could be classified as one group,and there was no obvious population differentiation.

关 键 词：圆鼻巨蜥 遗传多样性 RAPD 

分 类 号：Q95[生物学—动物学]