人α干扰素转基因小鼠模型的建立  

Establishment of Human Interferon-α Transgenic Mice Model

作　　者：李维纳[1] 衣帅[1] 刘烨[2] 扈荣良[2] 袁宝[1] 任文陟[1] 刘殿峰[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《安徽农业科学》2011年第13期7857-7859,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金重大专项"抗口蹄疫;抗乳房炎转基因克隆牛的培育"(2009ZX08007-007B)

摘　　要：[目的]建立人α干扰素(interferon-α,IFN-α)转基因小鼠,为研究IFN-α在抗病毒中的作用提供模型动物。[方法]将人IFN-α基因插入CMV启动子下游,构建转基因表达载体,通过原核显微注射法建立IFN-α转基因小鼠。[结果]通过特异性引物PCR法和South-ern杂交法检测出4只阳性转基因小鼠。分离4个转基因鼠系F1代PCR阳性小鼠血液中的淋巴细胞进行RT-PCR检测,其中3个鼠系为阳性。收集阳性小鼠血清,用ELISA方法和微量板染色测定法检测出3个转基因鼠系均有IFN-α表达。[结论]成功建立了CMV启动子启动的表达人IFN-α基因转基因小鼠,为研究干扰素抗病毒机制及对其他动物进行抗病毒感染基因工程育种研究奠定了基础。[Objective] To establish human interferon-α（IFN-α） transgenic mice,which can be used to study antiviral effect of IFN-α.[Method] The expression vector was constructed by inserting the human IFN-α gene into a vector harboring CMV promoter.The transgenic mice were created by the method of microinjection.The genotype of transgenic lines was identified by PCR and southern blotting.[Result] Four lines of transgenic mice of IFN-α were established.Lymphocytes of PCR-positive F1 generation mice were collected and identified by RT-PCR,and mice of three lines were positive.ELISA and microplate dye-binding assay showed the expression of human IFN-α in the serum of mice which were positive in RT-PCR identification.[Conclusion] The transgenic mice carrying CMV-controlled IFN-α gene has been established successfully,which is essential for studying antiviral effect of IFN-α and genetic engineering breeding of disease-resistant animals.

关 键 词：干扰素 转基因小鼠 CMV启动子 

分 类 号：S188.1[农业科学—农业基础科学]