快速康复外科干预对梗阻性黄疸大鼠肝脏缺血再灌注损伤疗效研究  

Study on the intervention of fast-track surgery on hepatic ischemical reperfusion injury in rats

作　　者：傅永清[1] 华晨[1] 陈炳荣[1] 张军[1] 王海娜[1] 周剑[1] 

机构地区：[1]浙江中医药大学附属第一医院外科,310006

出　　处：《浙江临床医学》2011年第5期487-490,共4页Zhejiang Clinical Medical Journal

基　　金：浙江省教育厅基金项目（No：Y201018053）

摘　　要：目的 探究快速康复外科对肝脏缺血再灌注损伤的干预作用.方法 健康SPF级SD大鼠60只,随机分成假手术组、非FTS组及FTS组,FTS组分常规组及中药干预组,每组各15只.所有大鼠均行胆总管结扎制备为梗阻性黄疸模型,FTS组及非FTS组均进行肝脏缺血再灌注操作.TFS中药组加用中药干预.24h后检测肝脏组织丙二醛（MDA）、超过氧化物歧化酶（SOD）,HE染色观察大鼠肝脏形态学改变,TUNEL法检测肝组织细胞原位凋亡,并计算凋亡指数（AI）.结果 各组间MDA比较无差异（P＞0.05）;假手术组与FTS常规组SOD无差异（P＞0.05）,其余各组相互比较均有差异（P＜0.05）;形态学观察：FTS组损伤情况轻于非FTS组;假手术组凋亡指数显著低于其他各组（P＜0.01）,非FTS组高于FTS组（P＜0.05）.结论 FTS的因素干预,改善了肝细胞的凋亡程度,降低了黄疸大鼠缺血再灌注损伤后肝细胞和肝功能的损伤程度.Objective To observe the effects intervention of fast -track surgery on hepatic ischemical reperfusion injury. Methods 60 SPF SD rats are randomly divided into the SHAM, the non FTS and the FTS, also the FTS is divided into FTS with herbal medicine and the general TFS. 15 in each group. All the rats underwent bile duct ligation for the model of jaundice. In the non FTS＆TFS, we induced hepatic isehemical reperfusion injury. In the FTS with herbal medicine we used herbal medicine. At last we detected theMDA,SOD. The morphologic changes and the apoptosis of the liver cell were observed. Results There was no difference between each group about the MDA （P 〉 0. 05）. About the SOD, there was no difference between the SHAM and the general FTS（P 〉 0. 05） , but in the others there were （ P 〈 0. 05 ）. For the morphologic changes, the FTS was lower. As for the apoptosis of the liver cell, the SHAM was lower than the others （ P 〈 0. 01 ）, the non FTS was higher than the FFS （ P 〈 0. 05 ）. Conclusions FTS will reduce the apoptosis of the liver cell and the injury of the hepatic cell ＆ the liver function after the hepatic ischemical reperfusion injury.

关 键 词：快速康复外科 肝脏缺血再灌注损伤 MDA SOD 细胞凋亡 

分 类 号：R657.3[医药卫生—外科学]