猪Reg3基因的克隆与序列分析  

Molecular Cloning and Sequence Analysis of Porcine Gene Reg3

作　　者：李文林[1] 陈福伟[1] 王伟杰[1] 韩立强[1] 王月影[1] 李宏基[1] 刘红亮[1] 杨国宇[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002

出　　处：《华北农学报》2011年第2期55-57,共3页Acta Agriculturae Boreali-Sinica

基　　金：国家科技攻关计划子课题(2006BAD04A03-10)

摘　　要：在猪的肠道组织中克隆Reg3基因,提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD-19T载体连接后转化E.coliJM109,检测阳性克隆、测序并进行序列分析。结果表明:克隆的猪Reg3基因与人、绵羊、马的同源性分别为83.7%,82.3%,82.2%;克隆了猪Reg3全长基因并注册GenBank注册号为Accession.FJ531494。In order to clone and analyze the gene Reg3 in intestinal canal tissues of pig,the total RNA was extracted and mRNA sequence of Reg3 gene was amplified by RT-PCR method which used two designed primers.The PCR productswere ligated into the pMD-19T vector,and then transformed into competent cells of E.coli JM109.The sequence was analyzed to identify the recombinant plasmid.The identification analysis showed that the Reg3 nucleotide sequence shared 83.7%,82.3% and 82.2% homology with that of human,sheep and horse respectively,revealing porcine Reg3 gene had been successfully cloned in the present study.The sequence has been submitted to GenBank（Accession FJ531494）.

关 键 词：猪 Reg3基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]