禁食对小鼠孤啡肽及其受体基因在脂肪组织中表达的影响  

Effects of Fasting on Nociceptin/Orphanin FQ and Opioid Receptor-like 1 mRNA Levels of Adipose Tissue in Mice

作　　者：陈福伟[1] 李文林[1] 韩立强[1] 李宏基[1] 郭豫杰[1] 王伟杰[1] 鲁维飞[1] 谢佳喜[1] 闫威[1] 杨国宇[1] 

机构地区：[1]河南农业大学农业部动物生化与营养重点实验室,河南郑州450002

出　　处：《华北农学报》2011年第2期81-84,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家科技攻关计划子课题(2006BAD04A03-10)

摘　　要：探讨禁食状态下孤啡肽及其受体mRNA在脂肪组织中表达的变化。将18只雄性昆明小鼠随机分为正常组、禁食组、再喂食组,采集其脂肪组织,用RT-PCR方法检测孤啡肽及其受体mRNA水平的变化。结果表明,孤啡肽及其受体基因均在小鼠脂肪组织中有表达,与正常组相比,禁食组孤啡肽及其受体的mRNA表达水平明显上调(P<0.05),而再喂食组表达差异不显著。说明在禁食情况下,孤啡肽及其受体基因对脂肪组织起到一定的调节作用。To observe effects of fasting on Nociceptin/Orphanin FQ（N/OFQ）and opioid receptor-like 1 mRNA（ORL1）levels of adipose tissue.18 mice were randomly divided into normal group,fasting group and re-feeding group.Adipose tissue was isolatd and analyzed by reverse transcription polymerdse chain reaction（RT-PCR）.N/OFQ and ORL1 gene were all expressed in adipose tissue.Compared with normal group,the mRNA levels of N/OFQ and ORL1 in fasting group increased significantly（P0.05）,but the mRNA levels in re-feeding group has no significant difference.N/OFQ and ORL1 may play a role in the regulation of metabolism in adipose tissue.

关 键 词：孤啡肽 受体 RT-PCR 禁食 脂肪组织 

分 类 号：Q786[生物学—分子生物学]