山羊体外成熟卵母细胞去核时间及方法对去核效率的影响  

The Effect of Enucleation Time and Method on the Enucleation Efficiency Using in vitro Matured Goat Oocytes

作　　者：刘海军[1] 郑梓[1] 王儒[1] 张金龙[1] 

机构地区：[1]天津市畜牧兽医研究所,天津300112

出　　处：《华北农学报》2011年第2期90-93,共4页Acta Agriculturae Boreali-Sinica

基　　金：天津市科技支撑计划重大项目资助(08ZCZDNC02100)

摘　　要：在山羊卵母细胞体外成熟的不同时间,进行荧光(Hochest 33342)染色,一是确定极体和细胞核染色体的位置关系,二是分别采用盲吸法和荧光指导法去核,观察去核率。结果表明:在体外成熟培养18,20,22,24 h,A类卵母细胞所占比例分别为72.7%,43.7%,27.5%,16.7%,B类卵母细胞分别为9.1%,31.3%,47.8%,55.6%,C类卵母细胞分别为18.2%,25%,24.6%,27.7%;在体外成熟培养的18,20,22,24 h去核,盲吸法的去核率分别为69.1%,58.2%,50.7%,45.8%,而荧光指导法去核率均为100%。Oocytes were stained with Hochest 33342 at the different time during in vitro maturing of goat oocytes,The first aim was to deterimine the location relationships between polybody and nucleus chromosome;the second aim was to compare the enucleation efficiency when using two different enucleation methods：blind-aspiration and fluorescence guiding.The result showed that at the 18,20,22,24 h during in vitro maturing of goat oocytes,the rate of type A oocytes was 72.7%,43.7%,27.5%,16.7%,type B oocytes was 9.1%,31.3%,47.8%,55.6%,type C oocytes was 18.2%,25%,24.6%,27.7%.When enucleating at the 18,20,22,24 h during in vitro maturing of goat oocytes,the enucleation efficiency were 69.1%,58.2%,50.7%,45.8% for blind-aspiration method,however,all the enucleation efficiency were 100% when fluorescence guiding method used.

关 键 词：山羊 卵母细胞 去核 

分 类 号：S827.8[农业科学—畜牧学]