拟南芥耐低钾突变体筛选标准的优化  

Optimization of the Method for Screening Arabidopsis low-K^+ Tolerant Mutants

作　　者：沈晓艳[1] 姜翠茹[1] 王增兰[1] 

机构地区：[1]山东师范大学山东省逆境植物研究重点实验室,山东济南250014

出　　处：《河南农业科学》2011年第3期56-58,共3页Journal of Henan Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2010CM0367)

摘　　要：为了探讨筛选耐低钾突变体的最优条件,比较了5种琼脂粉(糖)及不同钾浓度的低钾培养基对拟南芥生长的影响。结果表明:添加Seakem LE agarose培养基的钾离子浓度为43.07μmol/L;钾离子浓度在60、80、100、120、160μmol/L时,拟南芥弯根长度显著或极显著高于在40μmol/L时,因此,40μmol/L左右可作为耐低钾突变体的筛选标准。In order to find the optimal condition for screening Arabidopsis mutants with low K＋ tolerance,the effects of different kinds of agars and different concentrations of K＋ were compared on root growth of Arabidopsis.The results showed that Seakem LE agarose containing 43.07μmol/L of K＋.40 μmol/L K＋ should be the best choice to solidify K＋-free MS medium for screening low-K＋ tolerant mutant compared with other concentration,such as 60,80,100,120,160μmol/L.

关 键 词：拟南芥 耐低钾突变体 筛选条件 钾离子 

分 类 号：Q945.7[生物学—植物学]