西瓜全雌基因连锁的SRAP分子标记  

Identification of SRAP Molecular Markers Linked to Gynoecious Loci in Citrullus lanatus(Thunb.) Mansfeld

作　　者：朱小茜[1] 孙治强[1] 李晓慧[2] 徐小利[2] 

机构地区：[1]河南农业大学,河南郑州450002 [2]河南省农业科学院园艺所,河南郑州450002

出　　处：《河南农业科学》2011年第3期105-108,共4页Journal of Henan Agricultural Sciences

基　　金：国家现代产业技术体系建设专项项目(CARS-26)

摘　　要：采用SRAP技术探寻与西瓜全雌基因连锁的分子标记。以全雌母本和弱雌父本为双亲杂交获得F1,自交获得F2群体,用分离群体分组分析法(bulked segregant analysis,BSA)构建全雌和弱雌2个基因池,筛选957对引物。结果发现,只有引物me16-em20能在两池之间扩增得到2条特异带。经F2代单株验证,结果表明该特异带能稳定出现。以MAPMAKER(Version 3.0)软件分析,2个标记与全雌性位点的连锁距离分别为18.4 cM和11.4 cM。In this paper,SRAP technique was used to study the molecular markers related to gynoecious gene of watermelon.Gynoecious watermelon and monoecious watermelon were used as parents to get F1 and F2 was obtained by self-pollination of F1.2 DNA pools of Gynoecious and monoecious were built by Bulked Segregant Analysis（BSA） and 957 pairs of primers were screened.The results showed that 2 characteristic bands were obtained only by primer me16-em20.By the test of which primer was used to amplify the individual DNAs of the F2 population,the result indicated that this marker could appear steadily.Linkage analysis by the software of MAPMAKER（Version 3.0） indicated that their genetic distance to the gynoecious loci was18.4cM and 11.4cM respectively.

关 键 词：西瓜 全雌 分子标记 相关序列扩增多态性 

分 类 号：S651[农业科学—果树学]