玉米抗草甘膦基因(sxglr-11)的构建与表达  

Construction and Expression of Anti-Glyphasate Gene(sxglr-11)

作　　者：王艳梅[1] 王陆军[1] 白建荣[1] 赵明[2] 

机构地区：[1]山西省农业科学院作物科学研究所,山西太原030032 [2]山西省农业科学院,山西太原030006

出　　处：《山西农业科学》2011年第5期389-391,407,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省青年基金项目(2009021032-2);山西省农业科学院科技攻关项目(YGG0849)

摘　　要：将现有的抗草甘膦基因(sxglr-11)和带有Ubiquitin启动子的载体进行连接,用壮观霉素(spectino-mycin)抗生素标记,构建携带抗草甘膦基因(sxglr-11)的大肠杆菌。然后利用三亲杂交法,将构建的真核表达载体转入根瘤农杆菌菌株LBA4404。对菌落进行PCR检测以及质粒双酶切产物进行电泳检测,并用构建的工程菌转化玉米试管苗。研究结果表明,表达载体成功转入根瘤农杆菌LBA4404。Glyphosate-resistant gene existing （sxglr-11 ）and vector carrying the Uhiquitin promoter were connected and marked with speetinomycin （spectinomycin） antibiotic marker, to construct gene carrying glyphosate （ sxglr- 11 ） in the coliform bacilli. Later, with three-parental mating method, the eukaryotic expression vector transferred into Agrobacterium tumefaciens strain LBA4404. The products of mycelium PCR, plasmid PCR and plasmid digested by EcoR I and BamH I enzyme were tested by electrophoresis, and the constructed project Agrobacterium was cultured with plantlet of maize. The result showed that eukaryotic expression vector had been transformed into A grob acte rium tumefacie ns LBA4404.

关 键 词：玉米 真核表达载体 根癌农杆菌 抗草甘膦基因 

分 类 号：S513[农业科学—作物学]