用花粉管通道法将抗旱耐盐基因导入玉米自交系的研究  

Study on Transferring Gene of Salt Tolerance and Drought Resistance into Corn Inbred Line by Pollen Tube Path

作　　者：董春林[1] 张明义[1] 林忠平[2] 张彦芹[1] 杨丽莉[1] 梁改梅[1] 孙洁[1] 

机构地区：[1]山西省农业科学院旱地农业研究中心,山西太原030006 [2]北京大学生命科学学院,北京100871

出　　处：《山西农业科学》2011年第5期392-394,共3页Journal of Shanxi Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2009ZX08003-005B)

摘　　要：采用改进的花粉管通道法,将海藻糖合酶基因(TPS)导入玉米自交系郑58中。3～5叶期时喷雾Basta(0.7%)溶液筛选,进行PCR检测,结果表明,目的基因已导入转化植株并整合到植株染色体上。田间初步抗旱性检测显示,转化植株(株系)的抗旱性高于对照植株。应用改进的花粉管通道法导入抗旱目的基因,可以得到玉米抗旱新资源,是一种切实有效的抗旱育种新方法。Using the improved pollen tube path method, trehalose synthase gene （TPS） was transferred into corn inbred line Zheng 58. In the 3 - 5 leaf stage, the selection of spraying Basta （0.7%）solution was combined with PCR test, the new gene had been imported into the plants and integrated into the plant chromosomes. The initial drought-tolerance investigation in fields con firmed that the drought-tolerance of transgenie plants was higher than the non-transformed. The results showed that the new corn drought resistance germplasm could be gained by pollen tube path, and it is a new feasible and effective way for corn drought resistance breeding.

关 键 词：玉米 花粉管通道技术 海藻糖合酶基因(TPS) 转基因 

分 类 号：S513.032[农业科学—作物学]