精制蟒蛇油延缓皮肤衰老的实验研究  

Experimental Research of Refined Python Oil in Delaying Skin Aging

作　　者：任薇[1] 龙彦纲[1] 黄国鑫[1] 武文[1] 梁海春[1] 赵学军[1] 苏子仁[1] 

机构地区：[1]广州中医药大学新药开发研究中心,广东广州510006

出　　处：《广州中医药大学学报》2011年第3期253-255,259,336,共5页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】观察精制蟒蛇油对D-半乳糖所致衰老小鼠皮肤组织的延缓衰老作用。【方法】选用昆明种小鼠,随机分为蟒蛇油高、中、低剂量组(中药高、中、低剂量组,剂量分别为10、5、2.5 mL/kg),维生素E组(剂量为50 mg/只),模型组和正常对照组;除正常对照组外,其他组均采用颈背部皮下注射D-半乳糖(剂量为1 g/kg)法复制亚急性衰老模型,同时各组皮肤涂布给药,连续42 d。取各组小鼠背部皮肤检测含水量、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,并进行组织病理学检查。【结果】模型组皮肤含水量及SOD活力降低,MDA含量升高,与正常对照组比较差异具有显著性意义(均P<0.01);中药高、中、低剂量组及维生素E组均可升高皮肤含水量和SOD活力,降低MDA含量,与模型组比较差异有显著性意义(P<0.01),并可改善皮肤组织的病理形态变化。【结论】精制蟒蛇油具有一定延缓皮肤衰老的作用,其机制与其能增加皮肤含水量和SOD活力,降低MDA含量有关。Objective To study the effect of refined python oil on delaying D-galactose-induced skin aging in mice.Methods Kunming mice were randomized into high-,middle-and low-dose python oil groups（in the dosage of 10.0,5.0,and 2.5 mL/kg respecitively）,model group,normal group and positive control group（vitamin E,50mg for each mouse）.Except for the normal group,mice in the other groups were given daily subcutaneous injection of D-galactose（1g/kg） on the mice nape and back to induce sub-acute aging,and simultaneously were given skin application of the corresponding drugs for 42 continuous days.After treatment,the mice nape and back skin was sampled for the examination of water content,superoxide dismutase（SOD） activity and malondialdehyde（MDA） content.The skin histological features were also examined.Results In the model group water content and SOD activity were decreased,and MDA content was increased（P〈0.01 compared with those in the normal group）.Water content and SOD activity were increased,MDA content was decreased,and the pathological changes of the skin were improved in python oil groups and positive control group（P〈0.01 compared with those in the model group）.Conclusion Python oil exerts certain effect on delaying skin aging,and its mechanism is probably related with the increase of water content and SOD activity,and with the decrease of MDA content

关 键 词：蟒蛇油/药理学 皮肤衰老/中药疗法 皮肤/病理学 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]