参环毛蚓细胞原代培养灭菌方法的研究  

Disinfection Methods for Primary Cell Culture of Pheretima aspergillum

作　　者：林小桦[1] 李薇[1] 侯雪芹[1] 李巧容[1] 韦健红[1] 李维[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《广州中医药大学学报》2011年第3期264-267,337,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:30772741);广东省科技计划项目(编号:2007B02071004);广东省科技计划项目(编号:2009B060700006);广州市科技计划项目(编号:2010Y1-C981)

摘　　要：【目的】选择最佳灭菌方法,建立参环毛蚓细胞原代培养实验体系。【方法】通过4因素和3水平的正交试验法考察不同浓度比例抗生素组合液对参环毛蚓细胞的灭菌效果,比较不同抗生素种类和浓度对参环毛蚓细胞原代培养的影响。【结果】以抗生素组合液200 U/mL青霉素钠+400μg/mL硫酸链霉素+300 U/mL硫酸庆大霉素+5μg/mL两性霉素B对组织块的灭菌效果最佳,10 d污染率为0%,且细胞能保持较好活性,并贴壁生长。【结论】组合式灭菌法是参环毛蚓细胞原代培养的最佳有效灭菌方法,且操作简捷易行。Objective To optimize the disinfection method for the establishment of primary cell culture system of Pheretima aspergillum（E.Perrier）.Methods L9（34） orthogonal test was carried out to compare the disinfection effect of the mixture composed of different kinds of antibiotics at various concentrations on the primary-culture cells of Pheretima aspergillum.Results The most effective antibiotic mixture consisted of benzylpenicillin sodium 200 U/mL,streptomycin sulfate 400 μg/mL,gentamycin sulfate 300 U /mL and amphotericin B 5 μg/mL.When cultured the cells with the above antibiotic mixture,the contamination rate within 10 days was 0%,and the cells kept good activity in adherence growth.Conclusion The combined disinfection method has been proved to be effective and feasible for the sterilization of the primary cell culture system of Pheretima aspergillum.

关 键 词：参环毛蚓 细胞培养 灭菌 

分 类 号：R282.3[医药卫生—中药学]