农杆菌介导棉花茎尖遗传转化体系优化  

Optimization of Agrobacterium-mediated Transformation System of Shoot Apex

作　　者：刘明月[1] 左开井[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2011年第2期25-31,共7页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10Z180);国家转基因重大专项(2009ZX08009-091B)

摘　　要：生根困难和转化效率较低是农杆菌介导棉花茎尖遗传转化体系的应用瓶颈。本研究以柯字棉312为研究材料,探讨茎尖遗传转化中植物生长调节剂、生根诱导时间、无菌苗型、针刺处理等对茎尖生根率、茎尖形态以及抗性苗产生率等的影响。结果表明,1.5 mg/L NAA能够有效提高生根率,促进茎尖伸长;生根诱导时间是生根率的重要影响因子;经多次针刺处理的正常苗抗性苗率较高;培养5 d的正常苗茎尖生长速度和状态优于黄化苗。Low root-inducing rate and transformation efficiency are the tough problems affecting Agrobacterium-mediated genetic transformation of cotton shoot apex system.Using shoot apex of Coker 312,this paper investigated the effect of plant growth regulators,root-inducing time,different kind of sterile seedlings and acupuncture on the root-inducing rate,transformation on efficiency and morphology.The result showed that 1.5 mg/L NAA could not only promote root-inducing rate but also accelerate its growing speed;Foot-inducing time was an important factor affecting root-inducing rate.In addition,acupuncture shoot apex could increase the occuring rate of Kan-risitant plant,and the transformation efficiency of the the shoot apex from 5 day sterile seedlings was higher than that from the etiolated ones.

关 键 词：棉花 农杆菌介导 茎尖 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]