右旋一叶萩碱对小鼠黑色素瘤B16增殖及细胞周期的影响  

Effect of virosecurinine on proliferation and cell cycle of mouse melanoma cell line B16

作　　者：李朋军[1] 沈伟哉[1] 叶文才[2] 苏晓朴[3] 孙艳[3] 

机构地区：[1]暨南大学医学院人体解剖教研室,广东广州510632 [2]暨南大学中药及天然药物研究所,广东广州510632 [3]广东药学院生命科学与生物制药学院,广东广州510006

出　　处：《暨南大学学报（自然科学与医学版）》2011年第2期151-154,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(81000923);广东省医学科学技术研究基金项目(B2009117);广东高校优秀青年创新人才培育项目(2009400)

摘　　要：目的:探讨右旋一叶萩碱对小鼠黑色素瘤B16细胞增殖和细胞周期的影响及其作用机制。方法:右旋一叶萩碱作用于小鼠黑色素瘤细胞B16,MTT法检测细胞增殖;流式细胞术检测细胞周期。结果:右旋一叶萩碱以时间和浓度依赖的方式抑制B16细胞生长;药物作用72 h IC50为63.34μmol/L;右旋一叶萩碱诱导B16细胞凋亡并显著减少S期、G2/M期细胞。结论:右旋一叶萩碱可以抑制黑色素瘤细胞的恶性增殖,其作用机制与诱导细胞凋亡、减少S期和G2/M期细胞有关。Aim：To investigate the effect and mechanism of virosecurinine on proliferation and cell cycle of mouse melanoma cell line B16.Methods： B16 cells were treated with virosecurinine in vitro.Cell proliferation was measured by MTT assay.Cell cycle was detected by flow cytometry.Results： Virosecurinine inhibited proliferation of B16 in a time and dose-dependent manner with an IC50 value of 63.34 μmol/L at 72 h.Virosecurinine induced apoptosis on B16 cells and decreased cells of S and G2/M stages.Conclusion：Virosecurinine can inhibit malignant proliferation of melanoma cells.The mechanism is associated with induced apoptosis and decreased cells of S and G2/M stage in cell cycle.

关 键 词：右旋一叶萩碱 黑色素瘤 凋亡 增殖 细胞周期 

分 类 号：R979.19[医药卫生—药品]