Ni^(2+)-Cd^(2+)联合染毒大鼠睾丸支持细胞和生精细胞的体视学分析  

Stereological analysis of Ni^(2+)-Cd^(2+) combined effects on Sertoli cells and germ cells in rat testes

作　　者：夏艳[1] 朱伟杰[1] 

机构地区：[1]暨南大学生命科学技术学院生殖免疫研究所,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2011年第2期160-164,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学基金项目(A04010449)

摘　　要：目的:应用体视学方法定量分析Ni2+、Cd2+对大鼠睾丸支持细胞和生精细胞的影响。方法:63只雄性SD大鼠随机均分为21组,每组3只,实验试剂CdCl2和NiSO4通过腹腔注射给药,对照组(A1、A2、A3),以等量生理盐水代替,实验组分为急性染毒组、染毒2月组、染毒4月组(B1~G1,B2~G2,B3~G3),每天给药的质量分数分别为:B1、B2、B3组:2.9 mg/kg Cd2+,C1、C2、C3组:5.8 mg/kg Cd2+,D1、D2、D3组:5 mg/kg Ni2+,E1、E2、E3组:50mg/kg Ni2+,F1、F2、F3组:2.9 mg/kg Cd2++5 mg/kg Ni2+,G1、G2、G3组:5.8 mg/kg Cd2++50 mg/kg Ni2+。组织学切片观察,病理附值评分,采用体视学图像分析方法对各组大鼠睾丸细胞进行定量分析。结果:与对照组比较,G1组、F1组、G2组附值评分显著升高(P<0.05);B1~G1组粗线期精母细胞与支持细胞的平均细胞比率显著减小(P<0.05);急性染毒B1组、D1组支持细胞体密度和截面密度,C1组、D1组数密度均高于对照组(P<0.05),而C1~G1组生精细胞体密度低于对照组(P<0.01);染毒2月G2组支持细胞的体密度和截面密度显著增加,生精细胞的体密度显著降低(P<0.01);染毒4月G3组支持细胞的体密度、截面密度均显著下降(P<0.01)。结论:Ni2+、Cd2+染毒可能引起睾丸支持细胞损伤但数量无显著改变,生精细胞数量显著减少,可能导致精子发生障碍。Aim：To quantitatively analyse the changes of Sertoli cells and germ cells caused by cadmium chloride（CdCl2） and nickel vitriol（NiSO4） combination in rat testes.Methods： Sixty-three male adult SD rats were divided into 21 groups（n=3） randomly including control groups（group A1,A2,A3） and treated groups（B1～G1,B2～G2,B3～G3）.The treated groups were treated for acute and chronic effects for 2 and 4 months respectively,and were accordingly given solutions containing Cd2＋ 2.9,Cd2＋ 5.8,Ni2＋ 5,Ni2＋ 50,Cd2＋ 2.9＋ Ni2＋ 5,Cd2＋ 5.8＋ Ni2＋ 50 mg/kg·day.While control groups were given equal volume of normal saline instead.Haematoxylin and eosin staining was used to observe testis changes,pathological evaluation rules for assignment score were made to evaluate the result,stereology techniques were applied to quantitatively analyse the histological structure of the testicular tissues.Results： Compared with control groups,pathologic assignment scores for group G1、F1、G2 were significantly increased（P〈0.05）;cell ratios of pachytene germ cells to Sertoli cells for group B2～G2 were decreased significantly（P〈0.05）.Sertoli cells volume density（VV） and numerical density in area（NA） of group B1,D1,G2,numerical density（NV） of group C1,D1 were all higher than those of control groups（P〈0.05）,except group G3 was lower（P〈0.01）.Germ cells VV of group C1～G1,G2,G3 were reduced significantly when compared with control groups（P〈0.01）.Conclusion：The pathological assignment scores suggest plummet changes on the testis injuries caused by combined treatment of CdCl2 and NiSO4,the stereological numbers show damaged Sertoli cells and decreased germ cells,which is indicative of spermatogenic disturbance.

关 键 词：NI2+ Cd2+ 支持细胞 生精细胞 睾丸 体视学 

分 类 号：R994.1[医药卫生—毒理学]