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作 者:范慧佳[1] 梅赞[1] 杨杰[1] 曹园[2] 王强[1]
机构地区:[1]中国药科大学,江苏南京210009 [2]江苏省中医院,江苏南京210036
出 处:《现代中药研究与实践》2011年第3期31-33,共3页Research and Practice on Chinese Medicines
基 金:国家"重大新药创新"科技重大专项资助项目(NO:20092X09308-04)
摘 要:目的建立卷柏及垫状卷柏的HPLC指纹图谱分析方法并进行穗花杉双黄酮的含量测定。方法采用HPLC方法测定24批不同产地的卷柏及垫状卷柏药材,分析两者的指纹图谱特征及穗花杉双黄酮的含量。结果以穗花杉双黄酮为主的不同的7个特征峰分别共同构成卷柏及垫状卷柏的HPLC指纹图谱概貌,卷柏及垫状卷柏中穗花杉双黄酮的含量分别为1.77~3.82%和0.45~0.85%。结论卷柏及垫状卷柏的HPLC指纹图谱相似,主要成分穗花杉双黄酮的含量相差较大。Objective To establish a method to analyze HPLC fingerprint chromatogram of Selaginella tamariscina and Selaginella pulvinate and to determine the content of amentoflavone.Methods HPLC was applied for establishment of fingerprints of Selaginell atamariscina and Selaginella pulvinate which from different origin to analyze their fingerprint characteristic and content of amentoflavone.Results The different characteristic seven peaks in the chromatogram consisted of the common pattern of Herba Selaginella tamariscina and Selaginlla pulvinate.Two important mutual fingerprint peaks were found in both of them,one of which is amentoflavone.The content of amentoflavone in Herba Selaginella tamariscina and Selaginella pulvinate was 1.77-3.82% and 0.45-0.85% separately.Conclusion The HPLC fingerprint chromatogram of Selaginella tamariscina and Selaginella pulvinate was similar,but their were very different in the content of amentoflavone.
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