HPLC测定补肾活血胶囊中异秦皮啶的含量  

Determination of Isofraxidin in Bu Shen Huo Xue Capsules by HPLC

作　　者：陈维静 齐海峰 

机构地区：[1]吉林通化振国药业有限公司,吉林通化134100 [2]辽宁九州龙跃药业有限公司,辽宁沈阳110016

出　　处：《现代中药研究与实践》2011年第3期70-72,共3页Research and Practice on Chinese Medicines

摘　　要：目的建立高效液相色谱法测定补肾活血胶囊中异秦皮啶的含量。方法采用HPLC法测定制剂中异秦皮啶的含量。色谱柱:Kromasil TM C18柱(4.6 mm×150 mm,5μm);流动相:甲醇-水(30:70);流速:1 mL/min;检测波长:344 nm。结果异秦皮啶在0.020～0.200μg范围内线性良好(r=0.999 9),平均回收率为100.10%,RSD为0.36%。结论本方法操作简便、准确,可用于补肾活血胶囊中异秦皮啶的测定。Objective To establish an HPLC method for determination of isofraxidin in Bu Shen Huo Xue Capsules. Methods In this method Kromasil TM C18 column （4.6mm×150mm, 5μm） was used, methanolwater（30 ： 70） was used as a mobile phase .The detection wave-length was set at 344nm. Results The linear range were 0.020-0.200 μg （r=0.999 9）. The average recovery was 99.74% with RSD 0.42%. Conclusion This method is simple and the result is reliable.

关 键 词：高效液相色谱法 补肾活血胶囊 异秦皮啶 含量测定 

分 类 号：R286.0[医药卫生—中药学]