云南蜂胶中多酚化合物的HPLC分析研究  

Study on the Active Composition of the Propolis Extract from Yunnan by HPLC

作　　者：赵娜[1] 毕玉芬[1] 李园[2] 丁仲鹃[2] 戴云[1] 

机构地区：[1]云南民族大学化学与生物技术学院民族药资源化学国家民委-教育部重点实验室,云南昆明650521 [2]昆明医学院口腔医院,云南昆明650021

出　　处：《云南民族大学学报（自然科学版）》2011年第3期209-212,共4页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(30471225);"十一五"国家科技支撑计划项目(2006BAD27B09;2006BAD04A12);国家高技术研究发展计划项目(2007AA10Z320)

摘　　要：建立了利用高效液相色谱法同时测定提取物中6种多酚化合物的方法，利用冷浸超声法制备蜂胶乙醇提取物；以绿原酸为标准，在765nm波长下，用Folin—Ciocaheus法测定提取物中的总酚酸含量；以芦丁为标准，在510nm波长下测定提取物中总黄酮含量；对采自云南3个地区的蜂胶样品进行HPLC分析．结果表明：云南不同产地蜂胶中总酚酸含量在6％～16％之间；总黄酮含量在10％～24％之间，其70％乙醇提取物中松属素和柯因的含量较高．This paper builds the method that determines simultanceously six kings of phenolic compounds in tesing extraction by high - efficient liquid chromatography, to prepare the extration alcohol extraction by cold leaching ultrasound in ethanol. Standardizing chlovogenic acids, it detemines the content of total phenolic acids of extraction by Folin - Ciocaltaus Method at the wavelength 745 nm, determines the content of total flavonoid of extraction at the wavelength 510 nm. And it take HPLC analysis to 3 samples of propolis from different regions in Yunnan. The result shows that the content of toatl phenolic acid of propolis from different producing areas is 6%-16% ;the total flavoncing reaches 10%--24%, and the content of pinocembrin and shrysin are high in 70% alcohol extraction.

关 键 词：蜂胶 冷浸超声提取 酚酸 黄酮 高效液相色谱法 

分 类 号：O652.1[理学—分析化学]