氢化物-无色散原子荧光光谱法测定血液中的硒  

Determination of Selenium in Human Blood by Hydride Generation-Atomic Fluorescence Spectrometry

作　　者：何小青[1] 李攻科[1] 廖祝华[2] 

机构地区：[1]中山大学化学与化工学院,广州510275 [2]桂林工学院测试中心,桂林541004

出　　处：《深圳大学学报（理工版）》1999年第2期89-93,共5页Journal of Shenzhen University(Science and Engineering)

摘　　要：采用氢化物－原子荧光光谱法测定人体血液中硒的含量．健康者（30人）血液中硒的平均含量为114．6±11．2ng·mL^（－1）；肿瘤及心脏病患者（20人）血液中硒的平均含量为77．4±11．1ng·mL^（-1）。研究结果表明：肿瘤及心脏病患者血液中硒的含量明显低于正常人．该方法的回收率为90．5％－107．0％，相对标准偏差为6．6％，检出极限为0．65ng·mL（-1）．The determination of selenium in human blood by hydride - generation - atomic fluoresecence spectrometry (HG -AFS) is studied. HNO_3 - HClO_4 - HCl is used for sample pretreatment. The recoveries are from 90. 5% to 107. 0%, the detection lindt is 0. 65ng·mL^(-1), and the relative standard deviation (RSD) is 6.6%, Under optimized experimental conditions, selenium in human blood has been determined, The average concentration of Se is 114.6±11 .2ng·mL^(-1) for thirty healthy people, and 77 .4 ±11. 2ng·^(-1) for twenty patients. The resultS showed that the concentrafion of Se in the blood of a patient is lower than tha in a healthy person, and can be used for the clinical diagnosis.

关 键 词：氢化物 原子荧光光谱法 硒 血液 测定 

分 类 号：Q592.1[生物学—生物化学] R446.112[医药卫生—诊断学]