饥饿胁迫对南方鲇幼鱼糖代谢的影响  

Effects of Starvation on Metabolism of Glucose in Juvenile Silurus meridonalis

作　　者：范国燕[1] 李英文[1] 

机构地区：[1]重庆师范大学生命科学学院重庆市动物生物学重点实验室重庆市生物活性物质工程研究中心,重庆400047

出　　处：《重庆师范大学学报（自然科学版）》2011年第3期22-27,共6页Journal of Chongqing Normal University:Natural Science

基　　金：重庆市动物生物学重点实验室科研项目(2009)

摘　　要：在(20±1)℃条件下,以饥饿0周作为对照,分别测定了饥饿处理0、1、2、4和8周后南方鲇(Silurus meridona-lis)幼鱼的肝指数、糖原含量、血糖浓度及己糖激酶(HK)、葡萄糖激酶(GK)酶活性。结果显示,实验鱼随饥饿时间的延长,肝指数、糖原含量均呈明显下降趋势;饥饿1周后的肝指数为(2.44±0.36),肝糖原含量为(35.20±2.25)mg.g-1,肌糖原含量为(30.69±10.24)mg.kg-1,均开始显著低于对照(p<0.05);其中肝指数与肝糖原变化趋势相似。血糖浓度至饥饿4周时开始显著低于初始水平(p<0.05),表现出较好的稳定性。己糖激酶(HK)、葡萄糖激酶(GK)活性均在饥饿4周后开始显著低于初始水平(p<0.05),两者表现出相似的变化趋势。研究推测,南方鲇幼鱼应对饥饿胁迫时,优先动用肝脏内的储存物质肝糖原来提供能量;肝糖原含量与维持血糖的稳定有直接关系;饥饿可降低HK、GK活性,但短暂饥饿2周对两者活性无显著影响。To investigate the effects of starvation with different time courses on hepatosomatic index（HSI）,glycogen,serum glucose and activities of glycolytic key enzymes（HK、GK） in juvenile Silurus meridonalis.The fish were starved for 0,1,2,4,8 weeks respectively at（20±1） ℃.Samples were taken after the process.The starvation for 0 week is set as the control group.The results showed that,as starvation continued,HSI and glycogen decreased significantly.Fasting for 1 week,the HSI was（2.44±0.36） mg·g-1,the liver glycogen was（35.20±2.25） mg·g-1 and the muscle glycogen was（30.69±10.24）mg·kg-1 respectively;all of the three indices were significanely below original value（p〈0.05）.During the experiment,HSI took on a similar trend with liver glycogen.Fasting for 4 weeks,serum glucose had began to significanely below original value（p〈0.05）,but it had a lesser variation extent.The activities of HK and GK had began to significanely below original value after fasting for 4 weeks（p〈0.05）,and the two indices had a similar trend.So,it is suggested that during the starvation,the fish mainly use glucide as energy source,mobilize the substances in liver firstly and the change of liver glycogen has a direct relationship with the stability of serum glucose;and the starvation can restrain the activities of HK and GK,whereas,short-time starvation（two weeks） has a trivial effect.

关 键 词：南方鲇 饥饿 糖代谢 

分 类 号：Q955[生物学—动物学]