细胞间黏附分子-1在多柔比星肾病大鼠中的表达及意义  

Expression and significance of intercellular adhesionmolecule-1 in doxorubicin iduced nphropathy rat

作　　者：王丽敏[1] 罗巧华[2] 张静[2] 

机构地区：[1]佳木斯大学第一附属医院儿科,黑龙江佳木斯154003 [2]佳木斯大学,在读研究生黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2011年第2期6-7,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:观察细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)在多柔比星肾病大鼠肾组织中的表达,探讨ICAM-1在微小病变肾病综合征(MCNS)尤其是蛋白尿中的作用机制。方法:雄性Wistar大鼠85只随机分为正常组(40只)、肾病组(45只)。肾病组单次尾静脉注射多柔比星5mg/kg,建立MCNS模型,正常组注射等量生理盐水。于造模后第7d、14d、28d、42d测定各组大鼠24 h尿蛋白、血清白蛋白、胆固醇、甘油三酯、肌酐及尿素氮含量,同时采用免疫组织化学染色检测肾组织中ICAM-1的表达。结果:1肾病组大鼠尿蛋白排泄量逐渐增加,且各时期与正常组比较明显升高,均有显著性差异(P<0.01);2肾病组大鼠肾组织中ICAM-1表达较正常组明显增加,有显著性差异(P<0.01);3肾病组肾组织中I-CAM-1的表达与24h尿蛋白的排泄量呈正相关(r=0.919,P<0.01)。结论:ICAM-1在MCNS肾组织中表达明显增多,它的异常表达与蛋白尿的发生密切相关。Objective：To observe the expression of intercellular adhesion molecule-1（ICAM-1） in the renal tissue in doxorubicin induced nephropathy rats and investigate the role of ICAM-1 in proteinuria in minimal change nephrotic syndrome（MCNS）.Methods：Eighty five male Wistar rats were randomly divided into normal group（n=40） and nephropathy group（n=45）.The MCNS model of the nephropathy group was established by a single tail vein injection of doxorubicin 5mg/kg.The normal group was given 5mg/kg saline injection.At the end of the 7,14,28 and 42 days,all rats 24h urine protein,serum albumin,serum cholesterol,triglycerides,serum creatinine and serum urea nitrogen levels were detected and at the same time the ICAM-1 expression in renal tissue was detected by immunohistochemical staining.Results：①The urinary protein excretion of the nephritic rats gradually was increased,and compared with the normal group in different periods,there were significant differences（P0.01）;②The ICAM-1 expression in the renal tissue in the nephropathy group was higher than that in normal group,with a significant difference（P0.01）;③In the nephropathy group,the ICAM-1 expression was positively correlated to the 24 h urinary protein excretion（r=0.919,P0.01）.Conclusion：The ICAM-1 expression is increased significantly in the renal tissue in MCNS,and its abnormal expression is closely related to the occurrence of proteinuria.

关 键 词：多柔比星肾病 微小病变肾病综合征 细胞间黏附分子-1 蛋白尿 

分 类 号：R692[医药卫生—泌尿科学]