Apelin抑制小鼠成骨细胞MC3T3-E1凋亡  

Apelin Suppressed Apoptosis of Mouse Osteoblastic Cell Line MC3T3-E1

作　　者：邓春雷[1,2] 张磊屹[1] 吕国华[1] 唐四元[3] 

机构地区：[1]中南大学湘雅二医院脊柱外科,湖南长沙410011 [2]湖南省宁乡县人民医院内分泌科 [3]中南大学护理学院

出　　处：《实用预防医学》2011年第4期581-584,共4页Practical Preventive Medicine

基　　金：国家自然科学基金项目(30872708)

摘　　要：目的观察Apelin对小鼠成骨细胞MC3T3-E1凋亡的作用。方法 ELISA和吖啶橙/溴乙啶(AO/EB)染色法检测细胞凋亡,Western blot检测细胞Bcl-2和Bax表达以及cytochrome c的分泌,通过荧光素(AFC、AMC)标记的底物来检测活性。结果 Apelin抑制无血清饥饿诱导的MC3T3-E1细胞凋亡;Apelin抑制无血清饥饿诱导的cyto-chrome c的分泌和caspase-3、caspase-8c、aspase-9的活化;Apelin抑制地塞米松或肿瘤坏死因子-α(TNF-α)诱导的MC3T3-E1细胞凋亡。结论 Apelin可抑制成骨细胞凋亡。Objective To observe the effect of apelin on the apoptosis of mouse osteoblastic cell line MC3T3-E1. Methods ELISA and acridine orange/ethidium bromide（AO/EB） staining were used for detection of cell apoptosis.Western blot was applied to detection of cytochrome c release.Fluorescein（AFC or AMC）-tagged substrate was used for detection of caspase-3/8/9. Results Apelin suppressed serum-deprivation-induced apoptosis of MC3T3-E1 cells.Apelin suppressed serum-deprivation-induced cytochrome c release and caspase3,caspase-8,caspase-9 activation in MC3T3-E1 cells.Apelin suppressed MC3T3-E1 cell apoptosis induced by dexamethasone and tumor necrosis factor-α（TNF-α）. Conclusion Apelin can inhibit osteoblast apoptosis.

关 键 词：APELIN 成骨细胞 凋亡 

分 类 号：R332[医药卫生—人体生理学]