快速检测乙型肝炎病毒C基因启动子联合点突变  

Detection of core promoter mutation by a rapid method

作　　者：王战会 戴炜[2] 侯金林 冯筱榕 陈金军 孙剑 章廉 骆抗先 

机构地区：[1]第一军医大学第一附属医院传染科,广州510515 [2]深圳市东湖医院

出　　处：《中华医学检验杂志》1999年第5期290-292,共3页

基　　金：国家自然科学基金;广东省自然科学基金

摘　　要：目的　研究聚合酶链反应限制性片段长度多态性分析（ Ｐ Ｃ Ｒ Ｒ Ｆ Ｌ Ｐ） 法检测乙型肝炎病毒（ Ｈ Ｂ Ｖ） Ｃ 基因启动子区 Ｔ１７６２／ Ａ１７６４ 联合点突变的有效性。方法　将４４ 份慢性乙型肝炎患者的血清 Ｐ Ｃ Ｒ Ｒ Ｆ Ｌ Ｐ结果同测序结果进行综合分析。结果　在４４ 份慢性乙型肝炎患者的血清中，有１２ 份血清 Ｐ Ｃ Ｒ Ｒ Ｆ Ｌ Ｐ的结果为单纯突变株感染；１０ 份为突变株与野生株混合感染；２２ 份为单纯野生株感染，与测序结果相吻合。结论　 Ｐ Ｃ Ｒ Ｒ Ｆ Ｌ Ｐ检测 Ｈ Ｂ Ｖ／ Ｃ 基因启动子区 Ｔ１７６２／ Ａ１７６４ 联合点突变具有快速、简便以及敏感性高、特异性强等优点，且可用于观察 Ｔ１７６２／ Ａ１７６４ 变异株的动态变化。但是此法也有一定的局限性。Objective To confirm the efficiency of detecting the double point mutations in the core promoter sequence of HBV using mismatched PCR in combination with a restriction fragment length polymorphism assay (PCR RFLP). Methods 44 serum samples from chronic hepatitis B patients were analyzed through PCR RFLP and sequencing assay.Results In 44 serum samples, 12 were mutant HBV infection, 10 infected by mixed wild type and mutant virus, and 22 infected by wild type virus. It was identical with the sequencing results.Conclusions T1762/A1764 double point mutations could be detected rapidly, easily, sensitively and specifically using PCR RFLP. Meanwhile, this method can be used to confirm the relations between mutants and serum status of HBeAg. But this method also has some limitations.

关 键 词：乙型肝炎病毒 C基因启动子 联合点突变 

分 类 号：R512.62[医药卫生—内科学]