我国部分地区土壤中的苏芸金芽孢杆菌和球形芽孢杆菌  

SURVEY OF BACILLUS THURINGIENSIS AND BACILLUS SPHAERICUS FROM SOILS OF FOUR PROVINCES OF CHINA AND THEIR PRINCIPAL BIOLOGICAL PROPERTIES

作　　者：李荣森[1] 戴顺英[1] 李小刚[1] 罗成[1] 盛竹莓 孙明 

机构地区：[1]中国科学院武汉病毒研究所

出　　处：《微生物学报》1990年第5期380-388,共9页Acta Microbiologica Sinica

摘　　要：从云南、贵州、四川和陕西4省的土壤中分离到大量苏芸金芽孢杆菌(Bacillus thuringie-msis)和球形芽孢杆菌(Bacillus sphaeticus)菌株。血清型分析表明,苏芸金芽孢杆菌分离株分属于23个血清型中的13个血清型,另有近20%的自凝型菌株及部分与所有标准菌抗血清无反应的菌株。对该两种昆虫病原细菌的生态分布规律进行了分析。研究了全部苏芸金芽孢杆菌分离株对鳞翅目、鞘翅目及双翅目的6种昆虫的毒力特性、伴孢晶体与芽孢的形态,以及晶体蛋白质成分。观察和测定了球形芽孢杆菌分离株的形态和毒力,并分析了部分菌株的晶体蛋白质成分。得到22株高效苏芸金芽孢杆菌和2株高效球形芽孢杆菌。证明苏芸金芽孢杆菌是典型的土壤微生物类群,我国西南地区土壤中的苏芸金芽孢杆菌资源十分丰富。A number of isolates of Bacillus thurin-gtensis and Bacillus sphaericus were obtainedfrom soils of Southwestern Area and ShaanxiProvince of China.Among isolates of B.thuringiensis were under 13 sorts of serotypein total of 23 sorts of B.thuringiensis andabout 20% of auto-agglutinate strains.Rulesof ecologic destribution of two sorts of bac-teria were analysed.Toxicities on six speciesof insects,morphology and crystal proteinsof B.thuringiensis,as well as toxicities,mor-phology and crystal proteins of B.sphaericus,were investigated.22 strains of more efficientof B.thuringiensis and 2 strains of more ef-ficient of B.sphaericus were ■tained.It wasshown that B.thuringiensis is actually soilmicroorganism,and resource of B.thurin-giensis is much fruitful in Southwestern Areaof China.

关 键 词：苏芸金杆菌 球形芽孢杆菌 

分 类 号：S482.7[农业科学—农药学]