热休克蛋白70与家兔颈总动脉球囊损伤后增殖细胞核抗原表达的关系  

The relationship between expression of HSP70 and PCNA in ballooned rabbit carotid

作　　者：郭远林[1] 王瑞英[1] 任江华[1] 曹茂银[1] 宋长杰[1] 

机构地区：[1]湖北医科大学附属第二医院心内科,武汉430071

出　　处：《中国病理生理杂志》1999年第10期880-883,共4页Chinese Journal of Pathophysiology

摘　　要：目的：研究热休克蛋白70（HSP70）与经皮冠状动脉腔内成形术后再狭窄形成中细胞增生的关系。方法：复制家兔颈总动脉球囊损伤模型，用免疫组化方法检测术后3、9、14、28d损伤动脉壁中HSP70和增殖细胞核抗原（PC－NA）表达水平；用计算机图像分析系统定量，并对两者进行相关分析。结果：损伤动脉壁HSP70及PCNA检测呈阳性，正常动脉呈阴性。9d时两者表达指数均较各自3d时显著升高，14d均达峰值，28d显著降至3d时水平。时相相关分析两者显著正相关。结论：细胞增生活性升高伴随HSP70的产生增多；动脉损伤时机体可能通过HSP70合成增多而促进细胞再生，一定程度上促成再狭窄。To investigate the expression of heat shock protein 70 (HSP70) involving in the devefopmnt of resteno-sis after percutaneous transluminal coronary angioplasty (PTCA) - METHOD: The rabbit carotid balloon injury model wasused. Six animals were killed at 3 days, six at 9 days, sir at 14 days and sit at 28 days after injury repectively, and theballooned carotid segments were removed.then,immunohistochemistry combined with computer image analysis was used todetermine HSP70-expression index(HEI)and proliferating cell nuclear antigen(PCNA)-expression index(PEI)in injured arteries RESULTS:Both stainnings were negative in normal vessels while positive in injured vessels.HEI and PEIsignificantly increased at day 9 compared with day 3 and reached their maxiums at day 14, then declined at day 28 significantly.The time course of celllproliferation was significantly positive correlated with that of the HSP70-expression CON-CLUSION:Cell proliferation is accompanied by production of HSP70 .it seems that body may induce cell proliferation by in creasing the synthesis of HSP70,thus to accelerate the reairment of injured artery.

关 键 词：热休克蛋白 PCNA 颈总动脉 动脉球囊损伤 

分 类 号：R543.502[医药卫生—心血管疾病]