富血小板血浆对组织工程骨血管化的作用研究  

Role of platelet-rich plasma in vascularization of tissue engineering bone

作　　者：金芳[1] 羊书勇[2] 张建设[2] 郑维银[2] 李晨军[2] 朱宏卫[2] 李焰[2] 

机构地区：[1]南京医科大学第二附属医院耳鼻喉科,南京210011 [2]成都军区总医院附属口腔医院颌面外科

出　　处：《西南国防医药》2011年第5期482-484,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：成都军区总医院院管课题(07Y01)

摘　　要：目的探讨自体富血小板血浆(PRP)在骨缺损部位骨组织修复过程中局部血管化方面的作用。方法对新西兰大耳白兔进行自体PRP的制备,同时在双侧下颌角处制造骨组织缺损创伤模型,以骨组织缺损部位所加入移植材料的不同随机分为3组,A组(实验组):PRP+牛凝血酶+羟基磷灰石生物陶瓷(HA);B组(对照组):全血+牛凝血酶+HA;C组(空白组)骨缺损部位不作任何处理,直接原位周围软组织缝合。分别在术后第2、4、8 w观察局部新生血管化情况。结果术后第2 w,A组和B组可见有大量的纤维结缔组织,其中A组可见较多新生血管,C组可见少量新生纤维结缔组织,但未见明显新生毛细血管;术后第4 w,A组和B组可见大量新生血管,但A组优于B组,C组仅见少量纤维结缔组织增生;术后第8 w,A组和B组骨缺损基本修复,A组血管数量较B组少。结论在促进骨组织缺损修复方面,PRP具有进一步加速局部血管化形成的作用。Objective To investigate the role of platelet - rich plasma （PRP）in the local vaseularization during the repair of bone defect. Methods Autologous PRP from New Zealand white rabbits were prepared, and a traumatic model of bone defect at bilateral angles of mandible was established at the same time. Animals were randomly divided into three groups according to the material transplanted into bone defect site. Group A （ experimental group） was added with PRP ＋ bovine thrombin ＋ hydroxyapatite （ HA）, group B （control group）with whole blood ＋ bovine thrombin ＋ HA, and group C （blank group）without any treatment except the suture of surrounding soft tissues. Local vascularization was observed 2,4 and 8 w after transplantation. Results 2 w after transplantation, there were a large amount of fibrous connective tissue in group A and B, but only a little in group C ; many new vessels in group A, but no evident vascularization in group C were found. 4 w later, there were a large amount of new vessels in group A and B, but group A was superior to group B;less fibrous connective tissue in group C was found. 8 w later,bone defects in group A and B were almost repaired although group A showed less vessels than group B. Conclusion During the repair of bone defect, PRP can accelerate local vascularization further.

关 键 词：富血小板血浆 骨缺损 修复 血管化 

分 类 号：R687.34[医药卫生—骨科学]