长期大强度运动大鼠脑组织阿黑皮素原mRNA表达的变化  

Effects of long term and high intensity exercise on pro-opiomelanocortin gene expression in rat brain

作　　者：陈家旭[1] 杨维益[1] 梁嵘[1] 黄启福[1] 刘晓兰[1] 董浩 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中国病理生理杂志》1999年第10期925-927,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金!39430140

摘　　要：目的和方法：采用生物素标记阿黑皮素原（POMC）cRNA探针（Bio－cRNA）原位来交法结合图象分析，从基因转录水平现农长时期（共7周）大强度（速度由15m／min递增至35m／min，运动时间为20-25min/d）跑台运动的大鼠不同防区PONCmRNA表达。结果：该强度下大鼠末次运动结束后POMC基因表达在额叶皮层、海马CA1下降（前者至运动结束后3h仍未恢复，后者至运动结束后对30min即恢复），在下丘脑则增强。结论：POMCmRNA表达在该强度下运动的大鼠不同脑区呈现不同的动态变化趋势。In situ hybridizahon using biotin-labeled pro-opiolnelanocortin (POMC) cRNAprnbe, and integrating image patterm analysis were used to observe the effect of long term (total 7 weeks) and high intensitytreadmill exereise (speed from 15 m/min gradually increaswh to 35 m/min with 0 degree, 20 to 25 min/d duration) on thechangs of POMC mRNA expression in different areas of rat brain. RESULTS: Post-exercise instanily, the expression ofPOMC mRNA was decreased in frontal cortex, in which it was not recovery yet at post-exercise 3 hours; also decrad inhippeampal CA1, in which it was recovery at pet - exereise 30 whnutes. But the expression of POMC mRNA was increasedin hypothalama CONCLUSION: The dynamic changes of POMC gene expression in different areas of rat brain responding tosuch long term and high intensity exercise were not the same.

关 键 词：前阿片 黑素细胞 皮素激素 MRNA 应激 POMC 

分 类 号：R338[医药卫生—人体生理学] G804.21[医药卫生—基础医学]