17-DMAG对缺氧肿瘤细胞的辐射敏感性影响  

Effects of Radiation Sensitivity of 17-DMAG on Hypoxic Carcinima Cells

作　　者：吴晓芬[1] 洪承皎[1] 张保国[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院中心实验室江苏省放射医学与防护重点实验室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2011年第1期6-8,12,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(10275083)

摘　　要：目的研究17-DMAG对缺氧模拟剂氯化钴(CoCl_2)诱导的人宫颈癌HeLa细胞的辐射敏感性影响。方法首先应用不同浓度的CoCl_2处理HeLa细胞24h,采用Western blot技术检测HIF-1α蛋白表达量,然后选取浓度为200μmol/L的CoGl_2对Hela细胞进行模拟缺氧处理24 h,应用不同浓度的17-DMAG预处理细胞16h后,接受2、4、6、8Gy不同剂量的射线照射,采用克隆形成实验观察细胞的存活率,应用Western blot技术检测HIF-1α蛋白表达。结果在CoCl_2浓度为200μmol/L时HIF-1a蛋白表达量最强;不同浓度17-DMAG处理组的HeLa细胞在不同照射剂量组间的存活率差异均有高度统计学论(均P<0.01);17-DMAG预处理缺氧HeLa细胞后,HIF-1a蛋白表达随药物浓度增加受到显著抑制。结论 17-DMAG预处理可增加缺氧细胞辐射敏感性,其机制可能是通过抑制HIF-1a蛋白表达而发挥作用。Objective To investigate the radiosensitivity effect of 17-DMAG on human HeLa cells of cervical cancer,which was induced by Hypoxia mimetic cobalt chloride（CoCl_2）.Methods First, HeLa cells was treated by different concentrations of CoCl_2 for 24 hours.And HIF-1αprotein level was detected by Western blot analysis.Then the HeLa cells was dealed with CoCl2 in concentration of 200μmol/L.Cells were pre-treated with different concentrations of 17-DMAG 16h after receiving different doses of 2,4,6,8 Gy irradiation.The method of colony-forming was used to detect the radiation sensitivity. HIF-la protein level was detected by Western blot analysis.Results When CoCl2 was in concentration in 200μmol/L,the HIF-1a protein expression was highest;HeLa cells were cultured in different concenreations of 17-DMAG and exposed to different doses of Irradiation,the cell servival rate was significant（P〈0.01）;After 17-DMAG pretreatment hypoxia of HeLa cells,HIF-1a protein expression with the drug concentration were inhibited significantly.Conclusions 17-DMAG pretreatment hypoxia of HeLa cells can enhance radiosensitization and the mechanism may be through inhibition of HIF-1a protein expression.

关 键 词：17-DMAG 氯化钴 HIF-1Α 辐射敏感性 HELA细胞 

分 类 号：R811.5[医药卫生—放射医学]