青蒿琥酯增敏对宫颈癌HeLa细胞裸鼠移植瘤凋亡的影响  

Effects of Artesunate Combining with Radiation on Apoptosis in Nude Mice Transplanted with HeLa Cells of Cervical Cancer

作　　者：周媛媛[1] 封阳[1] 曹建平[1] 张旭光[2] 朱巍[1] 倪倩影[1] 耿冲[1] 陈光烈[1] 罗居东[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院放射生物教研室江苏省放射医学与防护重点实验室,江苏苏州215123 [2]徐州市肿瘤医院,江苏徐州221002

出　　处：《苏州大学学报（医学版）》2011年第1期13-15,20,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30670632);长江学者和创新团队发展计划资助项目(IRT0849)

摘　　要：目的探讨青蒿琥酯增敏对宫颈癌HeLa细胞裸鼠移植瘤凋亡的影响。方法建立裸鼠移植瘤模型,待移植瘤体积平均增至大约(5 mm×5 mm×5 mm),将裸鼠随机分为对照组、单药组、单照组和联合组,单药组和联合组每只裸鼠给予青蒿琥酯100 mg.kg^(-1).d^(-1),对照组和单照组每只裸鼠给予等体积生理盐水,处理7 d,第7 d给药后单照组和联合组立即给予一次性^(60)Coγ射线10Gy照射,照后观察14 d,14 d后剥瘤,TUNEL法检测移植瘤组织中细胞的凋亡。结果联合组裸鼠移植瘤瘤质量较单照组明显减小[(0.64±0.11)g vs(1.31±0.58)g](P<0.05),抑瘤率达71.17%。联合组移植瘤组织中细胞凋亡数较单照组显著增大[(77.5±8.07)vs(48.80±6.71)](P<0.05)。结论青蒿琥酯能明显增加HeLa细胞裸鼠移植瘤对射线的放射敏感性,作用机制与其增强射线诱导移植瘤组织中细胞凋亡有关。Objective To investigate the effect of Artesunate combining with radiation on apoptosis in transplanted tumors.Methods HeLa cells were inoculated into the nude mice to develop a tumor model.Mice were randomized into four groups as the control group,the Artesunate group,the irradiation group and the combination group when average volume of tumor achieved about 5 mm×5 mm×5 mm. During the period of treatment,the volume of tumors was measured per 2 days.After 14 days treatment, the mice were killed and tumor tissues were harvest,the tumor size and weight were measured,tumor inhibitory rate calculated and TUNEL assay was used to analysis the apoptosis of tumor tissue.Results The tumor weight in combination group was significantly lower than that than in the irradiation group [（0.64±0.11）gvs（1.31±0.58）g]（P〈0.05）,the tumor inhibitory rate was 71.17%.The apoptosis in the combination group was obviously higher than that in the irradiation group[（77.5±8.07 ）%vs （48.80±6.71）%]（P〈0.05 ）.Conclusion Artesunate can dramatically increase the radiosensitivity of tumor model transplanted with HeLa cells of cervical cancer,the possible mechanism of radiosensitization of Artesunate is related to increasing apoptosis of tumor cells.

关 键 词：青蒿琥酯 放射增敏 凋亡 HELA细胞 

分 类 号：Q691[生物学—生物物理学] R114[医药卫生—卫生毒理学]