中空膜液相微萃取-HPLC法用于人血浆中水杨酸的测定  

Determination of Salicylic Acid in Human Plasma by Hollow Fiber Liquid Phase Microextraction-HPLC

作　　者：刘健艺[1] 孟恒[1] 程燕[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药学与临床研究》2011年第2期131-133,共3页Pharmaceutical and Clinical Research

摘　　要：建立灵敏、准确地测定人血浆中水杨酸浓度的HPLC法。样品经过酸化处理后,以甲苯-正辛醇（4：6,V/V）为萃取溶剂、NaOH水溶液（pH=10）为接收相进行中空纤维膜微萃取。在该条件下,血浆中水杨酸浓度在2－250 ng·mL^-1范围内,线性关系良好（r=0.9994）;检测限为0.7ng·mL^-1（S/N=3）;精密度（RSD）〈8%（n=5）;回收率为87.6%－102.0%。该方法灵敏、有效、所需有机溶剂少、富集效率高,适于血浆中水杨酸浓度的测定。A sensitive,accurate method to determine salicylic acid in human plasma by HPLC was developed.Using hollow fiber liquid phase microextraction as a sample preparation technique,the optimum experimental conditions were the following： binary-solvent of toluene：n-octanol（4∶6,V/V） as extraction solvent,HCl solution as donor phase,NaOH solution at pH 10 as acceptor phase.Under the optimum conditions,the linear range of salicylic acid was 2-250 ng·mL^-1（r=0.9994）,the limit of detection was 0.7 ng·mL^-1,relative recoveries were 87.6%-102.0% with RSD less than 8%.The method was sensitive and effective for determination of salicylic acid in human plasma that required little organic solvent and provided a high degree of enrichment.

关 键 词：中空纤维膜液相微萃取 高效液相色谱法 水杨酸 血浆 

分 类 号：R927.2[医药卫生—药学]