中华蜜蜂王浆蛋白MRJP5的结构分析与功能预测  

Structure Analysis and Function Prediction of Major Royal Jelly Protein 5 of Apis Cerana ceranas

作　　者：刘芳[1] 苏松坤[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310029

出　　处：《蜜蜂杂志》2011年第5期1-4,共4页Journal of Bee

基　　金：国家科技部863计划探索项目资助(No.2007AA10Z332);现代农业产业技术体系建设专项资金资助(nycytx-43-kxj4)

摘　　要：调用motif数据库、profile数据库和interproscan数据库,对中蜂王浆蛋白MRJP5进行了序列同源性分析和功能位点分析。结果表明:MRJP5是分泌蛋白,理论等电点为8.05,分子量为70 522。应用多种相关软件对MRJP5的二级结构和特殊结构进行了初步预测,结果显示:MRJP5存在α螺旋、β折叠和无规则卷曲,有3个可形成跨膜结构的片段,有卷曲螺旋和信号肽。MRJP5属于MRJPs大家族成员,与该家族其他成员序列有较高的相似性。MRJP5存在N端糖基化位点、蛋白激酶C磷酸化位点、酪蛋白激酶Ⅱ磷酸化位点、N端豆蔻酰化位点等。这些为该基因的功能研究提供了重要的依据。Sequence homology and functional sites analysis were made on major royal jelly protein 5 （MRJP5） through motif database, profile database and interproscan database. The results show that MRJP5 is a kind of secreted protein, with atheoretical pl of 8.05 and molecular weight of 70 522. The second and special structure were predicted preliminarily using a variety of related software, it showed that alpha helix, extend strand and random coil exits in MRJP5, which has coiled coil, signal peptide and three fragments which could be membrane structures. MRJP5 is one member of MRJPs family, and has higher simi- larity with other members. MRJP5 has N-glycosylation site, protein kinase C phosphorylation site, casein kinase phosphorylation site and N-myristoylation site etc. All the information above will play a vital basis for the study of function of the gene.

关 键 词：MRJP5蛋白 结构分析 功能预测 

分 类 号：S891.1[农业科学—特种经济动物饲养]