SR-BI过表达对J774细胞胆固醇转运的影响  

Effect of Overexpression of SR-BI on Cholesterol Trafficking in J774 Cells

作　　者：曾颖[1,2] 冯惊涛[2] 孙玉慧[3] 孙琳[2] 曾德星[2] 易光辉[2] 

机构地区：[1]南华大学护理学院,湖南衡阳421001 [2]南华大学心血管病研究所 [3]广东省江门出入境检验检疫局国际旅行卫生保健中心

出　　处：《中南医学科学杂志》2011年第1期10-13,共4页Medical Science Journal of Central South China

基　　金：湖南省教育厅重点基金资助项目(09A078)

摘　　要：目的用构建的人SR-BI(hSR-BI)真核表达载体转染J774细胞,观察细胞瞬时过表达SR-BI对J774细胞胆固醇转运功能的影响。方法用hSR-BI cDNA转染法转染J774细胞,用RT-PCR和Western blot检测各组细胞的SR-BI表达,优化转染条件;用液体闪烁计数法检测流入和流出细胞的[3H]胆固醇量,分析过表达SR-BIJ774细胞的胆固醇转运功能。结果与对照组和空载体转染的J774细胞相比,pcDNA3.1(-)+hSR-BI转染的J774细胞SR-BI表达升高;与空载体转染的J774细胞相比,pcDNA3.1(-)+hSR-BI转染的J774细胞的胆固醇双向转运功能增强。结论用hSR-BI真核表达载体pcDNA3.1(-)+hSR-BI成功转染了J774细胞,使细胞过表达SR-BI,过表达的hSR-BI具有促进胆固醇转运的作用。Objective To investigate the effect of overexpression of SR-BI on the cholesterol trafficking in J774 cells. Methods J774 cells were transiently transfected by cloned hSR-BI to express hSR-BI, an 82 kDa protein. The expression of SR-BI were detected by RT-PCR and Western blot. The influx and efflux of[ 3 H ] cholesterol from cells were determined by liquid scintillation counting. Results The human scavenger receptor class B type I （hSR-BI） cDNA was successfully transfected into J774 cells. The expression level of SR-BI was elevated in J774 cells transfected with pcDNA3.1 （ - ） ＋ hSR-BI compared to control and vector （ P 〈 0.05 ）. The function of cholesterol trafficking in J774 cells transfected with pcDNA3.1 （ - ） ＋ hSR-BI was reinforced compared to control and vector. Conclusion Overexpression of SR-BI can promote cholesterol trafficking in J774 cells.

关 键 词：SR-BI 单核/巨噬细胞 转染 过表达 胆固醇转运 

分 类 号：R541.4[医药卫生—心血管疾病]